Proteases for Recombinant Protein Fusion Tag Cleavage

One of the factors in choosing a recombinant protein expression platform is the option of which proteolytic fusion tag recognition site will be incorporated into the protein sequence. Additional factors may include subsequent removal of the protease as well as easy removal of the cleaved fusion tag.
Use the Protease Finder to identify individual proteases that can be used to cleave a distinct peptide bond within your specific protein or peptide sequence.

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E5144 Enterokinase from bovine intestine powder
E4906 Enterokinase from bovine intestine BioUltra, recombinant, expressed in E. coli, ≥20 units/mg protein, ≥95% (SDS-PAGE)
E0885 Enterokinase from porcine intestine lyophilized powder, ≥100 units/mg protein
E0632 Enterokinase from porcine intestine ≥0.5 units/mg solid
SRP3032 Enterokinase human recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC), cell culture tested
PRKE Enterokinase Removal Kit
F9302 Factor X Activated (Xa) from bovine plasma aqueous glycerol solution
H9916 HRV3C Protease recombinant, expressed in E. coli
T4455 TEV Protease
T7513 Thrombin from bovine plasma lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
T6884 Thrombin from human plasma lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)
T1063 Thrombin from human plasma lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)
T9326 Thrombin human BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)
RECOMT Thrombin CleanCleave Kit