Cytochrome P450s (CYPs)

The hepatic microsomal cytochrome P450 (often referred to simply as P450) proteins are members of a superfamily of monoxygenases that catalyze the oxidative metabolism of xenobiotics, which is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants. P450s are located primarily in the endoplasmic reticulum of liver tissue. Until recently, the difficulty of isolating and reconstituting native enzymes from human tissue has hampered their use for studies with well-defined in vitro drug metabolism systems. Sigma-Aldrich is pleased to offer listings of Cytochrome P450s in both microsome preparations as well as purified isoforms.

Applications:
  • Isozyme identification
  • Determination of kinetic parameters for substrates and inhibitors
  • Isolation and synthesis of rare and novel metabolites
  • Stereo- and/or regio-specific hydroxylation reactions
  • High-throughput in vitro screening

Cytochrome P450 Human Isozyme Microsomes

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C5614 Cytochrome P450 human 1A2 Isozyme Microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
C5740 Cytochrome P450 human 2E1 isozyme microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

Cytochrome P450 human recombinant microsome preparations

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E9788 Cytochrome P450 1A2 human recombinant, expressed in Saccharomyces cerevisiae
E9163 Cytochrome P450 2C19 human recombinant, expressed in Saccharomyces cerevisiae
E9663 Cytochrome P450 2C8 human recombinant, expressed in Saccharomyces cerevisiae
E9538 Cytochrome P450 2C9 human recombinant, expressed in Saccharomyces cerevisiae
E9413 Cytochrome P450 2D6 human
E9038 Cytochrome P450 3A4 human recombinant, expressed in Saccharomyces cerevisiae
E9288 Cytochrome P450 2E1 human recombinant, expressed in Saccharomyces cerevisiae

Cytochrome P450 human recombinant purified soluble

Sigma-Aldrich is pleased to offer new purified Cytochrome P450 (CYP450) isoforms for your research needs. Purified CYP450′s offer the advantages of minimizing potential interfering activities from microsomal or tissue preparations of CYP450′s. In addition, you gain greater flexibility in your experiments and applications in terms of optimization of component ratios of CYP450, NADPH-P450 reductase, and cytochrome b5, as needed.

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H5417 Cytochrome P450 2C19, human recombinant, expressed in E. coli
H5292 Cytochrome P450 2C19 I331V, human recombinant, expressed in E. coli
H4792 Cytochrome P450 2C9*1 (Wild type allele), human recombinant, expressed in E. coli
H4667 Cytochrome P450 2C9*2 (R144C), human recombinant, expressed in E. coli
H4542 Cytochrome P450 2C9*3 (I359L), human recombinant, expressed in E. coli
H5042 Cytochrome P450 3A4 human recombinant, expressed in E. coli
H4917 Cytochrome P450 3A5 human recombinant, expressed in E. coli

Cytochrome P450 Mass Spectrometry Kits

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4465863 CYP450 Extended Panel - 100 Assay Kit
4466004 CYP450 Extended Panel - Starter Kit
4445252 P450 Human Induction 100 Assay Kit
4445494 P450 Human Induction Starter Kit

Cytochrome P450 Microsomes

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C3735 Cytochrome P450 human 1A1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
C3860 Cytochrome P450 human 1B1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
C4982 Cytochrome P450 human 3A4 isozyme microsomes, with P450 reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
C4985 Cytochrome P450 human 4F2 isozyme microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
C5110 Cytochrome P450 human 4F3A isozyme microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
C5235 Cytochrome P450 human 4F3B isozyme microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

Cytochrome P450 Supporting Reagents

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C1427 Cytochrome b5 human recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)
C8113 Cytochrome P450 Reductase human recombinant, expressed in baculovirus infected insect cells
M7316 Monoamine Oxidase A human recombinant, expressed in baculovirus infected BTI insect cells
M7441 Monoamine Oxidase B human recombinant, expressed in baculovirus infected BTI insect cells

CypExpress Human Recombinant Cytochrome P450

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MTOXCE19A1 CypExpress Aromatase
MTOXCE1A2 CypExpress 1A2 Cytochrome P450 human
MTOXCE2B6 CypExpress 2B6 Cytochrome P450 human
MTOXCE2C19 CypExpress 2C19 Cytochrome P450 human
MTOXCE2C9 CypExpress 2C9 Cytochrome P450 human
MTOXCE2D6 CypExpress 2D6 Cytochrome P450 human
MTOXCE3A4 CypExpress 3A4 Cytochrome P450 human
MTOXCENULL CypExpress Control