Ubiquitination

Enzymes/Proteins

Product #

Description

Add to Cart

23-021-M UBE1 Protein, active, 10 µg Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.
23-022-M UbcH3 Protein, active, 10 µg Active, full length, recombinant human UbcH3. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-023-M SCF-Skp2 Protein Complex, Active, 10 µg Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-025-M UbcH4 Protein, active, 50 µg Active, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-026-M SCF-TrCP1 Protein Complex, Active, 10 µg Active complex of four (4) recombinant human enzymes: full length Skp1, full length Cul1, full length Rbx1, & βTrCP1 (amino acids 53-end). For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-029-M UbcH5a Protein, active, 50 µg Active, full length, recombinant human UbcH5a. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-030-M SCF-Fbw7 Protein Complex, Active, 10 µg Active complex of four (4) full length, recombinant human enzymes: Fbw7, Skp1, Cul1 & Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-032-M MDM2/CK1δ Protein, active, 10 µg Active, full length, recombinant human MDM2 (N-terminal GST-tagged) combined in vitro with CK1δ, active (Item # 14-520). For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-033-M MDM2 (c-Myc tagged) Protein, active, 10 µg Active, full length, recombinant human MDM2. N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-035-M UbcH5c Protein, active, 10 µg Active, full length, recombinant human UbcH5c. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-036-M UbcH6 Protein, active, 10 µg Active, full length, recombinant human UbcH6. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-041-M c-Cbl Protein, active, 10 µg Active, full length, recombinant human c-Cbl. N-terminal GST-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-044-M VHL Protein Complex, Active, 10 µg Active complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, & full length Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-046-M Parkin (c-Myc tagged) Protein, active, 10 µg Active, full length, recombinant human Parkin. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-047-M UbcH7 Protein, active, 250 µg Active, full length, recombinant human UbcH7. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-048-M Parkin Protein, active, 10 µg Active, full length, human recombinant human Parkin. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
23-051-M UbcH13/Uev1A Protein, active, 10 µg Active complex of two (2) full length, recombinant human enzymes: UbcH13 & Uev1A. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
23-058-M Smurf1, active
23-059-M TRAF6, active
23-060-M IKKγ
U9257 UbcH9 Conjugating Enzyme human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
U6253 Ubiquitin from bovine erythrocytes BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder
U5382 Ubiquitin human ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), essentially salt-free, lyophilized powder
U5507 Ubiquitin human recombinant, expressed in E. coli (N-terminal histidine tagged)
U5632 Ubiquitin−Agarose saline suspension
U9382 Ubiquitin-Carrier Protein H10 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli (histidine-tagged), buffered aqueous glycerol solution
U2508 Ubiquitin Carrier Protein H2 human
U2383 Ubiquitin Carrier Protein H3/CDC34 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain
U8632 Ubiquitin-Carrier Protein H5a, GST-tagged human ≥95% (SDS-PAGE), liquid
U8757 Ubiquitin-Carrier Protein H5b human ≥95% (SDS-PAGE), solution
U8882 Ubiquitin-Carrier Protein H5c human ≥95% (SDS-PAGE), recombinant, expressed in E. coli (GST-tagged), solution
U9132 Ubiquitin-Carrier Protein H7 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, liquid
U8382 Ubiquitin Conjugating Enzyme Fractions mammalian
U8507 Ubiquitin K48R human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, solid
U2133 Ubiquitin C-terminal Hydrolase-L3

Ubiquitination Inhibitors

Product #

Description

Add to Cart

U1632 Ubiquitin, Methylated from bovine erythrocytes semisynthetic, ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Ubiquitination Substrates

Product #

Description

Add to Cart

17-10388 Di-Ubiquitin (Lys63-linked) Recombinant Protein Native K48 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.
17-10408 Di-Ubiquitin (Lys48-linked) Recombinant Protein Native K48 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.
23-024-M p27 Protein complex, activated, 10 µg Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
23-027-M b-catenin Protein, activated, 10 µg Activated, full length, recombinant human β-catenin. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using GSK-3a (Item # 14-492). For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
23-028-M IkBa Protein, activated, 10 µg Activated, full length, recombinant human IκBα. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using IKKα, active. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
23-034-M p53 Protein, 10 µg Full length, recombinant human p53. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
23-039-M Smad7
23-042-M Src Protein, 10 µg Full length, recombinant human Src. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
23-049-M p38/JTV-1 Protein, 10 µg Full length, recombinant human p38/JTV-1. N-terminal cMyc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
23-055-M Cardiac Troponin I, 10 µg Full length, recombinant human Cardiac Troponin I. N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
S0439 SUMO-1 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli (GST-tagged)
U5379 Anti-Ubiquitin antibody produced in rabbit whole antiserum, lyophilized powder
U2258 Ubiquitin-7-amido-4-methylcoumarin solution (supplied in DMSO)