Purified Enzymes

Firefly luciferase is used analytically to measure very small quantities (down to femptomoles) of ATP. Our BioUltra enzyme is at least 98% pure, after chromatography and crystallization. Endogenous ATPase and nucleoside diphosphokinase, both interfering activities, have been nearly eliminated. We also offer a partially purified luciferase from the bioluminescent Photobacterium fischeri. When this bacterial luciferase oxidizes reduced flavin mononucleotide (FMN) in the presence of a fatty aldehyde, blue-green light is emitted. Our extract contains NADH- and NADPH-dependent FMN reductases and can be used as a coupled system to measure NADH or NADPH.

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L9506 Luciferase from Photinus pyralis (firefly) BioUltra, ≥98% (SDS-PAGE), lyophilized powder, 15-30×106 light units/mg protein
L9420 Luciferase from Photinus pyralis (firefly) recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
SRE0045 Luciferase from Photinus pyralis (firefly) recombinant, expressed in E. coli, lyophilized powder, ≥1×1011 units/mg protein
L8507 Luciferase from Vibrio fischeri (Photobacterium f) lyophilized powder