Trypsin Substrates

Kinetic measurement of trypsin activity is usually followed spectrophotometrically using arginine esters or amides as substrates. Cleavage of BAEE (Na-Benzoyl-L-arginine-ethyl-ester-hydrochloride) is measured in the ultraviolet range, while arginine nitroanilide and napthylamide substrates produce colored products when cleaved , and thus are useful in colorimetric assays. Several of these chromogenic substrates are tripeptides, altering their specificity. For example, Pefachrome Fxa is primarily used as a substrate for clotting factor Xa. Researchers can also follow amidase and esterase activity flurometrically, with 4-methylcoumarin and methylumbelliferin substrates respectively.

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11610   Azo-Casein protease substrate  
B7260 Nα-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride protease substrate 83701-04-6
B4500 Nα-Benzoyl-L-arginine ethyl ester hydrochloride trypsin substrate 2645-08-1
B0885 Nα-Benzoyl-L-arginine 4-methoxy-β-naphthylamide hydrochloride 100900-33-2
B4750 Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride trypsin substrate 913-04-2
B3279 Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride >98% (TLC) 21653-40-7
B3133 Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride ≥99% (TLC), suitable for substrate for trypsin 21653-40-7
B4875 Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride ≥98% 911-77-3
87528 Nα-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide ≥97.0% (HPLC) 59068-47-2
B7632 N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride protease substrate 54799-93-8
B4153 Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin hydrochloride ~95% 113866-20-9
39890   N,N-Dimethylcasein BioReagent, for spectrophotometric det. of enzyme activity  
H6268   Hide–Remazol Brilliant Blue R protease substrate 37340-54-8
51010 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate ≥85% (HPLC) 34197-46-1
77723 Pefachrome® Try ≥95.0% (HPLC) 161572-28-7
T4626 Nα-p-Tosyl-L-arginine methyl ester hydrochloride 1784-03-8