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E. coli Source

E. coli Source ß-Glucuronidases
ß-Glucuronidase is a ~290 kDa tetrameric protein with an isoelectric point of 4.8.1 Unlike the enzyme preparations from mollusks that naturally contain ß-glucuronidase and sulfatase activities in almost equal amounts, the preparation of ß-glucuronidase from E. coli is essentially free of sulfatase activity. The enzyme from E. coli has a high rate of hydrolytic activity and it retains this activity during hydrolysis better than similar enzymes that are more sensitive to changes in the concentration of ß-glucuronide conjugates. The enzyme preparation from E. coli has been shown to be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.2 The E. coli enzyme has also been shown to be more active against estrogen conjugates than other sources of the enzyme.3
Optimal pH: 6-7

1. Kim, D-H, et al., Biol. Pharm. Bull., 18, 1184-1188 (1995)
2. Graef, V., et al., Clin. Chem., 23, 532-535 (1977)
3. Jayle, M.F., Scholler, R., Jarrige, P. & Mtay, S. Bull. Soc. Chim. Biol. 41, 1593. (1959)

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BGALS-RO β-Glucuronidase from E. coli K 12
G7396 β-Glucuronidase from Escherichia coli Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
G7646 β-Glucuronidase from Escherichia coli Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
G8162 β-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)
G8295 β-Glucuronidase from Escherichia coli ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder
G8420 β-Glucuronidase from Escherichia coli ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder