Cultured cells must be passaged periodically to maintain their vigor and viability. Most commonly, researchers use trypsin, sometimes in combination with EDTA, to separate the adherent cells from their culture substrate and from each other. The amount of protease needed depends upon the cell type and the age of the culture. We offer trypsin from porcine pancreas in several strengths, and in a variety of media to optimize passaging conditions. Some formulations include the chelator EDTA. All have been sterile filtered and are thus ready-to-use. In addition, all solutions have been tested and are free of porcine parovirus.