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Trypsin Solutions for Cell Dissociation

Cultured cells must be passaged periodically to maintain their vigor and viability. Most commonly, researchers use trypsin, sometimes in combination with EDTA, to separate the adherent cells from their culture substrate and from each other. The amount of protease needed depends upon the cell type and the age of the culture. We offer trypsin from porcine pancreas in several strengths, and in a variety of media to optimize passaging conditions. Some formulations include the chelator EDTA. All have been sterile filtered and are thus ready-to-use. In addition, all solutions have been tested and are free of porcine parovirus.

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T4549 Trypsin solution from porcine pancreas 10 ×, sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride
T4674 Trypsin solution from porcine pancreas 10 ×, sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
T4424 Trypsin solution from porcine pancreas 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
T4049 Trypsin-EDTA solution 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
T3924 Trypsin-EDTA solution 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
T4299 Trypsin-EDTA solution 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA • 4Na per ml in Dulbecco′s PBS without calcium and magnesium
T4174 Trypsin-EDTA solution 10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA • 4Na per liter of 0.9% sodium chloride