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Trypsin for Protein Mass Spectrometry

Our highly purified porcine trypsin is available in two forms, under three product listings, to fit the protein chemist’s needs. In both forms, it has been methylated on its lysine residues to prevent autolysis, treated with TPCK (tosyl phenylalanyl chloromethyl ketone) to eliminate chymotryptic activity, and affinity purified. The enzyme comes as either a lyophilized powder (T6567, T7575) or covalently bound to treated silica particles in a convenient spin column (TT0010). The spin column, with its short incubation time of about 15 minutes, is ideal for small volumes. Complete digestion of proteins to tryptic peptides for sequencing or mass spectrometry requires that the protein first be denatured, as folded proteins are usually resistant to proteolysis. Denaturation is commonly accomplished by reduction and methylation, although the use of chaotropic agents such as urea may be more appropriate for some proteins.

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EMS0004 SOLu-Trypsin recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid New
T6567 Trypsin from porcine pancreas Proteomics Grade, BioReagent, Dimethylated
T7575 Trypsin Singles, Proteomics Grade
TT0010 Trypsin Spin Columns for proteomics