Polyacrylamide-based glycoconjugates have been reportly used as probes for cell surface carbohydrate binding proteins. They have been used for detection of cells using flow cytometry,1 and have been immobilized on microtiter plates for an immunosorbent assay method.2
1. Masking and unmasking of the sialic acid-binding lectin activity of CD22 (Siglec-2) on B lymphocytes. Razi, N., and Varki, A., Proc. Natl. Acad. Sci. USA., 95, 7469-7474 (1998).
2. Norwalk virus-like particle hemagglutination by binding to H histo-blood group antigens. Hutson, A.M., et al., J. Virology, 77, 405-415 (2003).
3. Carbohydrate-based probes for detection of cellular lectins. Galanina, O.E., et al., Anal. Biochem., 265, 282-9 (1998).
4. Chemically synthesized solid phase oligosaccharide probes for carbohydrate-binding receptors. Interactions of the E-, L- and P-selectins with sialyl-Le(x) and O-sulphated forms linked to biotin or to polyacrylamide. Pavlovic, D., et al., J. Immunol. Methods, 264, 53-8 (2002).