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Atto Labels

Atto labels are designed for high sensitivity applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, or enzyme assays. Secondary antibody, streptavidin, and biotin conjugates are available for most of the Atto labels.
  • Spectral data: Emission maxima cover a broad range from 500 to 700 nm
  • Stoke′s shift: Shift between absorption maxima and emission maximum is sufficient to discriminate emission from excitation
  • Atto labels exhibit bright fluorescence, extraordinary photostability and good hydrophilicity

Atto Label Technical Details

Product #

Description

Assay

Fluorescence

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89313 Atto 390 ≥98.0% (HPCE)
λex 390 nm; λem 475 nm in 0.1 M phosphate pH 7.0
89204 Atto 390 NHS ester ≥80% (coupling to amines)
λex 389 nm; λem 471 nm in 0.1 M phosphate pH 7.0
43378 Atto 390 alkyne   λex 390 nm; λem 479 nm±10 nm in 0.1 M phosphate pH 7.0
68321 Atto 390 azide ≥80.0% (HPCE)
λex 390 nm; λem 479 nm±10 nm in PBS, pH 7.4
89740 Atto 390 maleimide ≥80% (coupling rate)
λex 390 nm; λem 472 nm in 0.1 M phosphate pH 7.0
56759 Atto 425   λex 440 nm; λem 484 nm
16805 Atto 425 NHS ester   λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
78948 Atto 425 azide ≥80.0% (HPCE)
λex 436 nm; λem 484 nm±10 nm in PBS, pH 7.4
49349 Atto 425 maleimide ≥70% (coupling to thiols)
λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
06714 Atto 430LS ≥80.0% (HPCE)
λex 433 nm; λem 545 nm±10 nm in PBS, pH 7.4
56387 Atto 430LS NHS ester   λex 433 nm; λem 540.0 nm±10.0 nm in PBS, pH 7.4
41882 Atto 430LS maleimide   λex 433 nm; λem 540.0 nm±10 nm in PBS, pH 7.4
50712 Atto 465   λex 453 nm; λem 508 nm
53404 Atto 465 NHS ester ≥80% (HPCE)
λex 449 nm; λem 503 nm in 0.1 M phosphate pH 7.0
55607 Atto 465 maleimide ≥90% (coupling to thiols)
λex 452 nm; λem 502 nm in 0.1 M phosphate pH 7.0
41051 Atto 488 ≥90% (HPCE)
λex 504 nm; λem 521 nm in 0.1 M phosphate pH 7.0
41698 Atto 488 NHS ester ≥80% (coupling to amines)
λex 498 nm; λem 520 nm in 0.1 M phosphate pH 7.0
55444 Atto 488 alkyne   λex 501 nm; λem 523 nm±10 nm in 0.1 M phosphate pH 7.0
74417 Atto 488 amine   λex 501 nm; λem 523 nm
72709 Atto 488 azide   λex 501 nm; λem 523 nm
74402 Atto 488 iodoacetamide   λex 501 nm; λem 523 nm
28562 Atto 488 maleimide ≥90% (coupling to thiols)
λex 501 nm; λem 523 nm in 0.1 M phosphate pH 7.0
06715 Atto 490LS ≥80.0% (HPCE)
λex 496 nm; λem 661 nm±10 nm in PBS, pH 7.4
78362 Atto 490LS NHS ester   λex 496 nm; λem 644.0 nm±10.0 nm in PBS, pH 7.4
93688 Atto 490LS azide   λex 496 nm; λem 655.0 nm±10.0 nm in PBS, pH 7.4
78363 Atto 490LS maleimide   λex 496 nm; λem 640.0 nm±10 nm in PBS, pH 7.4
16951 Atto 495 ≥98.0% (HPCE)
λex 499 nm; λem 526 nm in 0.1 M phosphate pH 7.0
00379 Atto 495 NHS ester ≥90% (coupling to amines)
λex 499 nm; λem 524 nm in 0.1 M phosphate pH 7.0
41022 Atto 495 maleimide   λex 500 nm; λem 524 nm in 0.1 M phosphate pH 7.0
52595 Atto 514 ≥80.0% (HPCE)
λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4
67455 Atto 514 NHS ester   λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4
80758 Atto 514 alkyne   λex 511 nm; λem 533 nm±5 nm in 0.1 M phosphate pH 7.0
93728 Atto 514 amine   λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4
44311 Atto 514 azide   λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4
80027 Atto 514 iodoacetamide   λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4
81062 Atto 514 maleimide   λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4
70706 Atto 520 ≥90.0% (HPCE/HPLC)
λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
77810 Atto 520 NHS ester ≥80.0% (coupling to amines)
λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
61911 Atto 520 azide ≥80.0% (HPCE)
λex 516 nm; λem 545 nm±10 nm in PBS, pH 7.4
16590 Atto 520 maleimide ≥90% (coupling rate)
 
06699 Atto 532 ≥90% (HPCE)
λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0
88793 Atto 532 NHS ester ≥80% (coupling to amines)
λex 530 nm; λem 551 nm in 0.1 M phosphate pH 7.0
53403 Atto 532 alkyne   λex 532 nm; λem 553 nm±10 nm in 0.1 M phosphate pH 7.0
68499 Atto 532 maleimide ≥90% (coupling to thiols)
λex 532 nm; λem 553 nm in 0.1 M phosphate pH 7.0
40592 Atto 540 Q ≥98.0% (HPCE)
 
61683 Atto 540 Q NHS ester ≥90% (coupling to amines)
 
62453 Atto 540 Q maleimide ≥90% (coupling to thiols)
 
42424 Atto 550 ≥90% (HPCE)
λex 554 nm; λem 574 nm in 0.1 M phosphate pH 7.0
92835 Atto 550 NHS ester ≥80% (coupling to amines)
λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0
52998 Atto 550 alkyne   λex 554 nm; λem 576 nm±5 nm in 0.1 M phosphate pH 7.0
30730 Atto 550 maleimide ≥90% (coupling to thiols)
λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0
75784 Atto 565 ≥75% (sum of isomers, HPCE/HPLC)
λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
72464 Atto 565 NHS ester ≥70.0% (coupling to amines)
λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
51934 Atto 565 alkyne   λex 563 nm; λem 592 nm±5 nm in 0.1 M phosphate pH 7.0
18507 Atto 565 maleimide   λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
03722 Atto 580 Q    
44756 Atto 580 Q NHS ester ≥90% (coupling to amines)
λex 586 nm
68152 Atto 580 Q maleimide ≥90% (coupling to thiols)
 
70425 Atto 590 ≥90% (HPCE)
λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0
79636 Atto 590 NHS ester ≥60% (coupling to amines)
λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0
93990 Atto 590 alkyne   λex 594 nm; λem 624 nm±5 nm in 0.1 M phosphate pH 7.0
39887 Atto 590 maleimide   λex 594 nm; λem 624 nm (pH 7.0)
08637 Atto 594 ≥90.0% (HPCE)
λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
08741 Atto 594 NHS ester ≥80% (coupling to amines)
λex 597 nm; λem 625 nm in 0.1 M phosphate pH 7.0
52986 Atto 594 alkyne   λex 601 nm; λem 627 nm±10 nm in 0.1 M phosphate pH 7.0
61583 Atto 594 amine   λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
08717 Atto 594 maleimide ≥80% (coupling rate)
λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
78493 Atto 610 ≥85.0% (HPCE/HPLC)
λex 610 nm; λem 635 nm in 0.1 M phosphate pH 7.0
41061 Atto 610 maleimide ≥90% (coupling to thiols)
λex 610 nm; λem 637 nm in 0.1 M phosphate pH 7.0, 2-mercaptoethanol
93259 Atto 610-NHS ester ≥90.0% (coupling to amines)
λex 610 nm; λem 635 nm in 0.1 M phosphate pH 7.0
94464 Atto 611X maleimide ≥80% (coupling to thiols)
λex 611; λem 681 nm in 0.1 M acetate buffer pH 4.7
04327 Atto 612 Q ≥98.0% (HPCE)
 
53988 Atto 612 Q NHS ester ≥90% (coupling to amines)
λem 615 nm
50332 Atto 612 Q maleimide ≥90% (coupling to thiols)
 
92716 Atto 620 ≥90% (HPCE)
λex 617 nm; λem 641 nm in 0.1 M phosphate pH 7.0
67351 Atto 620 NHS ester ≥80% (coupling to amines)
λex 620 nm; λem 641 nm in 0.1 M phosphate pH 7.0
49728 Atto 620 maleimide ≥80% (coupling to thiols)
λex 619 nm; λem 643 nm in 0.1 M phosphate pH 7.0
18620 Atto 633   λex 633 nm; λem 657 nm in 0.1 M phosphate pH 7.0
01464 Atto 633 NHS ester ≥90.0% (coupling to amines)
λex 630 nm; λem 650 nm in 0.1 M phosphate pH 7.0
90729 Atto 633 alkyne   λex 629 nm; λem 657 nm±10 nm in 0.1 M phosphate pH 7.0
68517 Atto 633 azide ≥80.0% (HPCE)
λex 629 nm; λem 657 nm±5 nm in 0.1 M phosphate pH 7.0
68652 Atto 633 iodoacetamide   λex 629 nm; λem 655 nm in 0.1 M phosphate pH 7.0
08968 Atto 635 ≥90% (HPCE)
λex 635 nm; λem 659 nm in 0.1 M phosphate pH 7.0
43429 Atto 635 NHS ester ≥80% (coupling to amines)
λex 624 nm; λem 655 nm in 0.1 M phosphate pH 7.0
97875 Atto 647 ≥90% (HPCE)
λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0
07376 Atto 647 NHS ester ≥80% (coupling to amines)
λex 643 nm; λem 669 nm in 0.1 M phosphate pH 7.0
41784 Atto 647 maleimide ≥90% (coupling to thiols)
λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0
04507 Atto 647N ≥98.0% (HPCE)
λex 644 nm; λem 667 nm in 0.1 M phosphate pH 7.0
18373 Atto 647N NHS ester ≥85% (coupling to amines)
λex 647 nm; λem 661 nm in 0.1 M phosphate pH 7.0
95349 Atto 647N amine   λex 644 nm; λem 661 nm
91000 Atto 647N azide   λex 644 nm; λem 661 nm
73353 Atto 647N iodoacetamide   λex 644 nm; λem 661 nm
05316 Atto 647N maleimide ≥90% (coupling to thiols)
λex 644 nm; λem 669 nm in 0.1 M phosphate pH 7.0
93711 Atto 655 ≥85% (HPLC)
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
76245 Atto 655 NHS ester   λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
92009 Atto 655 alkyne   λex 663 nm; λem 684 nm±5 nm in 0.1 M phosphate pH 7.0
14918 Atto 655 amine   λex 663; λem 684 nm in 0.1 M phosphate pH 7.0
11774 Atto 655 azide   λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
73948 Atto 655 iodoacetamide   λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
80661 Atto 655-maleimide ≥90% (coupling rate)
λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
16851 Atto 665 (free acid) ≥95.0% (HPCE)
λex 663 nm; λem 684 nm±5 nm in 0.1 M phosphate pH 7.0
04022 Atto 665 NHS ester ≥90% (coupling to amines)
λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
01407 Atto 665 maleimide ≥90% (coupling to thiols)
λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
94875 Atto 680 ≥90% (HPLC)
λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0
75999 Atto 680 NHS ester ≥90.0% (coupling to amines)
λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0
04971 Atto 680 maleimide ≥90% (HPCE)
λex 680 nm; λem 700 nm in 0.1 M phosphate pH 7.0
30674 Atto 700 ≥96.0% (HPCE)
λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0
16986 Atto 700 NHS-ester ≥90% (coupling to amines)
λex 681 nm; λem 713 nm in 0.1 M phosphate pH 7.0
19571 Atto 700 amine   λex 700 nm; λem 719 nm in 0.1 M phosphate pH 7.0
59825 Atto 700 azide ≥80.0% (HPCE)
λex 700 nm; λem 740 nm±10 nm in PBS, pH 7.4
50611 Atto 700 maleimide ≥90% (coupling to thiols)
λex 645 nm; λem 715 nm in 0.1 M phosphate pH 7.0
47156 Atto 725 ≥98.0% (HPCE)
λex 725 nm; λem 752 nm in 0.1 M phosphate pH 7.0
93725 Atto 725 NHS ester ≥90% (coupling to amines)
λex 725 nm; λem 753 nm in 0.1 M phosphate pH 7.0
90979 Atto 725 maleimide ≥80% (coupling to thiols)
λex 725 nm; λem 752 nm in 0.1 M phosphate pH 7.0
91394 Atto 740 ≥98.0% (HPCE)
λex 740 nm; λem 764 nm in 0.1 M phosphate pH 7.0
59808 Atto 740 NHS ester ≥90.0% (coupling to amines)
λex 740 nm; λem 766 nm in 0.1 M phosphate pH 7.0
77071 Atto 740 maleimide ≥80.0% (coupling to thiol groups)
λex 740 nm; λem 764 nm in 0.1 M phosphate pH 7.0
75118 Atto MB2    
73499 Atto MB2 NHS ester    
51797 Atto MB2 maleimide    
02934 Atto MB2-Biotin    
92606 Atto Oxa12   λex 663 nm; λem 684 nm
61938 Atto Oxa12 Biotin   λex 663 nm; λem 684 nm
55785 Atto Oxa12 NHS ester   λex 663 nm; λem 684 nm
01971 Atto Oxa12 maleimide   λex 663 nm; λem 684 nm
77085 Atto Rho101   λex 586 nm; λem 610 nm
50492 Atto Rho101 NHS ester   λex 586 nm; λem 610 nm
73522 Atto Rho101 maleimide   λex 586 nm; λem 610 nm
56611 Atto Rho11   λex 571 nm; λem 595 nm
19096 Atto Rho11 Biotin   λex 571 nm; λem 595 nm
89101 Atto Rho11 NHS ester   λex 571 nm; λem 595 nm
44432 Atto Rho12   λex 576 nm; λem 601 nm
91254 Atto Rho12 Biotin   λex 576 nm; λem 601 nm
68761 Atto Rho12 NHS ester   λex 576 nm; λem 601 nm
72763 Atto Rho12 maleimide   λex 576 nm; λem 601 nm
16973 Atto Rho13   λex 600 nm; λem 625 nm
76141 Atto Rho13 Biotin   λex 600 nm; λem 625 nm
68538 Atto Rho13 NHS ester   λex 600 nm; λem 625 nm
44535 Atto Rho13 maleimide   λex 600 nm; λem 625 nm
59746 Atto Rho14   λex 625 nm; λem 646 nm
76140 Atto Rho14 Biotin   λex 625 nm; λem 646 nm
61909 Atto Rho14 NHS ester   λex 625 nm; λem 646 nm
14397 Atto Rho14 maleimide   λex 625 nm; λem 646 nm
93685 Atto Rho3B   λex 564 nm; λem 592 nm
07876 Atto Rho3B Biotin   λex 564 nm; λem 592 nm
28743 Atto Rho3B NHS ester   λex 564 nm; λem 592 nm
76531 Atto Rho3B maleimide   λex 564 nm; λem 592 nm
88821 Atto Rho6G   λex 535 nm; λem 560 nm
93516 Atto Rho6G NHS ester   λex 535 nm; λem 560 nm
36535 Atto Rho6G alkyne   λex 535 nm; λem 560 nm±10 nm in 0.1 M phosphate pH 7.0
87785 Atto Rho6G maleimide   λex 535 nm; λem 560 nm
94169 Atto Rho6G-Biotin   λex 535 nm; λem 560 nm
56963 Atto Thio12   λex 579 nm; λem 609 nm
87784 Atto Thio12 Biotin   λex 579 nm; λem 609 nm
94079 Atto Thio12 maleimide   λex 579 nm; λem 609 nm