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Atto Labels



Atto labels are designed for high sensitivity applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, or enzyme assays. Secondary antibody, streptavidin, and biotin conjugates are available for most of the Atto labels.
  • Spectral data: Emission maxima cover a broad range from 500 to 700 nm
  • Stoke's shift: Shift between absorption maxima and emission maximum is sufficient to discriminate emission from excitation
  • Atto labels exhibit bright fluorescence, extraordinary photostability and good hydrophilicity

Atto Label Technical Details

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89313Atto 390 ≥98.0% (HPCE)
λex 390 nm; λem 475 nm in 0.1 M phosphate pH 7.0
pricing
89204Atto 390 NHS ester ≥80% (coupling to amines)
λex 389 nm; λem 471 nm in 0.1 M phosphate pH 7.0
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89740Atto 390 maleimide ≥80% (coupling rate)
λex 390 nm; λem 472 nm in 0.1 M phosphate pH 7.0
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16805Atto 425-NHS ester λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
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49349Atto 425-maleimide ≥70% (coupling to thiols)
λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
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50712Atto 465 pricing
55607Atto 465 maleimide ≥98.0% (HPCE)
λex 452 nm; λem 502 nm in 0.1 M phosphate pH 7.0
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53404Atto 465-NHS ester ≥80% (HPCE)
λex 449 nm; λem 503 nm in 0.1 M phosphate pH 7.0
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41051Atto 488 ≥90% (HPCE)
λex 504 nm; λem 521 nm in 0.1 M phosphate pH 7.0
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28562Atto 488 maleimide ≥90% (coupling to thiols)
λex 501 nm; λem 523 nm in 0.1 M phosphate pH 7.0
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41698Atto 488-NHS ester ≥80% (coupling to amines)
λex 498 nm; λem 520 nm in 0.1 M phosphate pH 7.0
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16951Atto 495 ≥98.0% (HPCE)
λex 499 nm; λem 535 nm in 0.1 M phosphate pH 7.0
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41022Atto 495 maleimide λex 500 nm; λem 524 nm in 0.1 M phosphate pH 7.0
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00379Atto 495-NHS ester ≥90% (coupling to amines)
λex 499 nm; λem 524 nm in 0.1 M phosphate pH 7.0
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70706Atto 520 ≥90.0% (HPCE/HPLC)
λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
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77810Atto 520 NHS ester ≥80.0% (coupling to amines)
λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
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16590Atto 520-maleimide ≥90% (HPCE)
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06699Atto 532 ≥90% (HPCE)
λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0
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88793Atto 532 NHS ester ≥80% (coupling to amines)
λex 530 nm; λem 551 nm in 0.1 M phosphate pH 7.0
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68499Atto 532 maleinimide ≥90% (HPCE)
λex 532 nm; λem 553 nm in 0.1 M phosphate pH 7.0
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40592Atto 540 Q ≥98.0% (HPCE)
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61683Atto 540 Q NHS ester ≥90% (coupling to amines)
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62453Atto 540 Q maleimide ≥98.0% (HPCE)
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42424Atto 550 ≥90% (HPCE)
λex 554 nm; λem 574 nm in 0.1 M phosphate pH 7.0
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92835Atto 550 NHS ester ≥80% (coupling to amines)
λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0
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30730Atto 550 maleimide ≥90% (HPCE)
λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0; 2-mercaptoethanol
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75784Atto 565 ≥75% (sum of isomers, HPCE/HPLC)
λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
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72464Atto 565 NHS ester ≥70.0% (coupling to amines)
λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
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18507Atto 565-maleimide λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
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03722Atto 580 Q ≥98.0% (HPCE)
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44756Atto 580 Q NHS ester ≥90% (coupling to amines)
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68152Atto 580 Q maleimide ≥98.0% (HPCE)
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70425Atto 590 ≥90% (HPCE)
λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0
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79636Atto 590 NHS ester ≥60% (coupling to amines)
λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0
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39887Atto 590-maleimide pricing
08637Atto 594 ≥98.0% (HPCE)
λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
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08741Atto 594 NHS ester ≥80% (coupling to amines)
λex 597 nm; λem 625 nm in 0.1 M phosphate pH 7.0
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08717Atto 594 maleimide ≥80% (coupling rate)
λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
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78493Atto 610 ≥85.0% (HPCE/HPLC)
λex 610 nm; λem 635 nm in 0.1 M phosphate pH 7.0
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93259Atto 610 N-succinimidyl ester ≥90.0% (coupling to amines)
λex 610 nm; λem 635 nm in 0.1 M phosphate pH 7.0
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41061Atto 610-maleimide ≥98% (HPCE)
λex 610 nm; λem 637 nm in 0.1 M phosphate pH 7.0; 2-mercaptoethanol
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40049Atto 611X λex 611 nm; λem 673 nm in 0.1 M phosphate pH 7.0
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18708Atto 611X NHS ester ≥90% (coupling to amines)
λex 611 nm; λem 673 nm in 0.1 M phosphate pH 7.0
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04327Atto 612 Q ≥98.0% (HPCE)
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53988Atto 612 Q NHS ester ≥90% (coupling to amines)
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50332Atto 612 Q maleimide ≥98.0% (HPLC)
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92716Atto 620 ≥90% (HPCE)
λex 617 nm; λem 641 nm in 0.1 M phosphate pH 7.0
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67351Atto 620 NHS ester ≥80% (coupling to amines)
λex 620 nm; λem 641 nm in 0.1 M phosphate pH 7.0
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18620Atto 633 pricing
01464Atto 633 NHS ester ≥90.0% (coupling to amines)
λex 630 nm; λem 650 nm in 0.1 M phosphate pH 7.0
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08968Atto 635 ≥90% (HPCE)
λex 635 nm; λem 659 nm in 0.1 M phosphate pH 7.0
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43429Atto 635 NHS ester ≥80% (coupling to amines)
λex 624 nm; λem 655 nm in 0.1 M phosphate pH 7.0
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97875Atto 647 ≥90% (HPCE)
λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0
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07376Atto 647 NHS ester ≥80% (coupling to amines)
λex 643 nm; λem 669 nm in 0.1 M phosphate pH 7.0
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41784Atto 647 maleimide ≥90% (coupling to thiols)
λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0
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04507Atto 647N ≥98.0% (HPCE)
λex 644 nm; λem 667 nm in 0.1 M phosphate pH 7.0
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18373Atto 647N NHS ester ≥85% (coupling to amines)
λex 647 nm; λem 661 nm in 0.1 M phosphate pH 7.0
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05316Atto 647N maleimide ≥90% (couplinf to thiols)
λex 644 nm; λem 669 nm in 0.1 M phosphate pH 7.0
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93711Atto 655 ≥85% (HPLC)
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
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76245Atto 655 NHS ester λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
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80661Atto 655-maleimide ≥90% (coupling rate)
λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
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94875Atto 680 ≥90% (HPLC)
λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0
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75999Atto 680 NHS ester ≥90.0% (coupling to amines)
λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0
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04971Atto 680-maleimide ≥90% (HPCE)
λex 680 nm; λem 700 nm in 0.1 M phosphate pH 7.0
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30674Atto 700 ≥96.0% (HPCE)
λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0
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16986Atto 700 NHS-ester ≥90% (coupling to amines)
λex 681 nm; λem 713 nm in 0.1 M phosphate pH 7.0
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50611Atto 700 maleimide ≥98.0% (HPCE)
λex 645 nm; λem 715 nm in 0.1 M phosphate pH 7.0
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47156Atto 725 ≥98.0% (HPCE)
λex 725 nm; λem 752 nm in 0.1 M phosphate pH 7.0
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93725Atto 725 NHS ester ≥90% (coupling to amines)
λex 725 nm; λem 753 nm in 0.1 M phosphate pH 7.0
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91394Atto 740 ≥98.0% (HPCE)
λex 740 nm; λem 764 nm in 0.1 M phosphate pH 7.0
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59808Atto 740 NHS ester ≥90.0% (coupling to amines)
λex 740 nm; λem 766 nm in 0.1 M phosphate pH 7.0
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