Metal Complexes

with Long Lifetimes

This series of reactive dyes is for use in fluorescence polarization assays and fluorescence lifetime imaging applications. These laser excitable metal ligand complexes, based on ruthenium, have fluorescence lifetimes between 400 and 800 nanoseconds. Traditional fluorophores such as fluorescein and rhodamine have fluorescence lifetimes of less than 10 nanoseconds. Longer lifetimes enable both time gated as well as time-dependent measurement. Another important aspect is their utility for polarization assays with high-molecular weight antigens. They can also be used to label amines on biomolecules under mild conditions.

Structure

Product #

Description

Fluorescence

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90819 Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate) λex 458 nm; λem 612 nm in 0.1 M Tris pH 7.0
71603 Bis(2,2′-bipyridine)-(5-isothiocyanato-phenanthroline)ruthenium bis(hexafluorophosphate) λex 445 nm; λem ~615 nm in 0.1 M phosphate pH 7.0
96631 Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis(hexafluorophosphate) λex 458 nm; λem 628 nm in 0.1 M phosphate pH 7.0
  02816 Europium-tetracycline complex reagent λex 386 nm; λem 617 nm±5 nm (H2O2-bound)