Phycobili Proteins

Phycobili Proteins - Enhanced Sensitivity
Sigma offers activated, biotinylated or streptavidin linked phycoerythrin, phycocyanin, and CryptoFluor™ dyes. These dyes are the first choice for applications that require either high sensitivity or simultaneous multicolor detection, e.g. flow cytometry and immunoassay. Phycobili proteins exhibit both quantum yields near 100% and extremely high extinction coefficients. The fluorescence yield of a single molecule is equivalent to at least 30 fluorescein or 100 rhodamine molecules at comparable wavelengths. CryptoFluor dyes are phycobiliproteins isolated from cryptomonad algae that provide additional spectral characteristics to complement the more common phycobiliproteins (e.g., R-PE and APC) as well as providing much smaller molecular weights.
CryptoFluor™ is a trademark of Martek Biosciences Corporation, Columbia, MD, USA.

Product #

Description

Assay

Fluorescence

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44709 B-Phycoerythrin, activated   λex 542 nm; λem 576 nm in 0.1 M phosphate pH 7.2
75166 Biotin-B-Phycoerythrin   λex 542 nm; λem 576 nm in 0.1 M phosphate pH 7.0
52468 C-Phycocyanin   λex 609 nm; λem 643 nm in 0.1 M phosphate pH 7.2
52412 R-Phycoerythrin   λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2
P8912 R-Phycoerythrin from Porphyra tenera “Nori”   λex 488 nm; λem 578 nm(lit.)
92984 Streptavidin B-Phycoerythrin   λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.0
17866 Streptavidin−CryptoFluor Crimson   λex 585 nm; λem ~658 nm in 0.1 M phosphate pH 7.2