Rhodamine and Derivatives

Rhodamines are supplements to fluoresceins, as they offer longer wavelength emission maxima and provide opportunities for multicolor labeling or staining. DNA sequencing, microarray detection and fluorescence in situ hybridization (FISH) demand a number of fluorophores with distinct spectra. Rhodamines exhibit higher photostability than fluorescein. Isothiocyanates and succinimidyl esters are available for convenient labeling.

  • Tetramethylrhodamine (TMR): a common fluorophore for preparing protein conjugates, especially antibody and avidin conjugates
  • Carboxy tetramethyl-rhodamine (TAMRA): important for olignucleotide labeling and automated nucleic acid sequencing
  • Carboxy-X-rhodamine (ROX): important for oligonucleotide labeling and automated nucleic acid sequencing
  • Sulforhodamine 101: a common protein label for three-color applications

Product #

Description

Assay

Fluorescence

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21965 5(6)-Carboxy-X-rhodamine   λex 570 nm; λem ~595 nm in 0.1 M Tris pH 8.0
21966 5(6)-Carboxy-X-rhodamine N-succinimidyl ester   λex 575 nm; λem 605 nm in 0.1 M phosphate pH 7.0
21953 5(6)-Carboxytetramethylrhodamine ≥85% (sum of isomers, HPCE)
λex 543 nm; λem 572 nm in methanol
21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester ≥70% (coupling to amines)
λex 543 nm; λem 576 nm in methanol
λex 554 nm; λem 584 nm in 0.1 M phosphate pH 8.0
41969 5-Carboxy-X-rhodamine N-succinimidyl ester   λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0
53048 5-Carboxy-tetramethylrhodamine N-succinimidyl ester   λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0
C2734 5-Carboxytetramethylrhodamine   λex ~543 nm; λem ~570 nm in methanol
88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester   λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0
55222 6-Carboxytetramethylrhodamine ≥90% (HPLC)
λex 543 nm; λem 575 nm in 0.1 M Tris pH 8.0
05803 N-(2-Aminoethyl)rhodamine 6G-amide bis(trifluoroacetate) ≥95.0% (HPCE)
λex 528 nm; λem 553 nm in 0.1 M phosphate pH 3.0
00814 N-(6-Aminohexyl)rhodamine 6G-amide bis(trifluoroacetate) ≥95.0% (HPCE)
λex 528 nm; λem 553 nm in 0.1 M phosphate pH 3.0
42514 N-(8-Amino-3,6-dioxaoctyl)rhodamine 6G-amide bis(trifluoroacetate) ≥95.0% (HPCE)
λex 528 nm; λem 553 nm in 0.1 M phosphate pH 3.0
29297 N-[2-(2-Aminoethylamino)ethyl]rhodamine 6G-amide bis(trifluoroacetate) ≥90% (HPCE)
λex 528 nm; λem 553 nm in 0.1 M phosphate pH 3.0
42932 N-[4-(Aminomethyl)benzyl]rhodamine 6G-amide bis(trifluoroacetate) ≥95.0% (HPCE)
λex 528 nm; λem 553 nm in 0.1 M phosphate pH 3.0
90877 N-[trans-4-(Succinimidyloxycarbonyl)cyclohexylmethyl]-sulforhodamine B-acid amide ≥85.0% (coupling to amines)
λex ≥566 nm; λem ≥587 nm in 0.1 M phosphate pH 7.0
74171 N-Fmoc Rhodamine 110   λex 492 nm; λem 521.0 nm±10 nm in ethanol
283924 Rhodamine B isothiocyanate    
R1755 Rhodamine B isothiocyanate   λex 543 nm; λem 580 nm in methanol
S3388 Sulforhodamine 101 acid chloride   λex 586 nm; λem 605 nm in H2O
86186 Sulforhodamine B acid chloride   λex 560 nm; λem 580 nm in chloroform
λex 560 nm; λem ~580 nm in chloroform
87918 Tetramethylrhodamine isothiocyanate mixed isomers   λex 529 nm; λem 596 nm in DMSO
94506 Tetramethylrhodamine-5-maleimide ≥85% (HPLC)
λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0
92364 Tetramethylrhodamine-6-maleimide ≥50% (HPLC)
≥97% (isomer purity, HPLC)
λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.5