Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Nucleic Acid Stains For Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

Product #

Description

Add to Cart

32670 4′,6-Diamidino-2-phenylindole dihydrochloride BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
46065 Ethidium bromide for fluorescence, ≥95.0% (HPLC)
46067 Ethidium bromide solution for fluorescence, ~1% in H2O
46043 Ethidium homodimer suitable for fluorescence, ~90% (HPCE)