Protein Stains For Immunoassays

General Features & Benefits
  • Immunological methods are based upon the competitive binding of labeled ligands versus unlabeled ones on highly specific receptor sites
  • Very important tools for clinical and analytical biochemistry and the analysis of drugs, pesticides, hormones and proteins
  • Outstanding specificity and sensitivity, adaptability to automation & an ability to quantitate an almost limitless number of analytes.
  • Competitive (higher selectivity) and non-competitive (higher sensitivity) immunoassay design

Heterogeneous Immunoassays
  • Physical separation of bound from free labeled analyte. Mostly the analyte or antibody is attached to a solid surface
  • Detection can be direct (only a primary antibody is used) or indirect (a secondary antibody is also used)

Homogenous Immunoassays
  • Fluorescence polarization excitation of fluorochromes
  • Double-antibody fluorophore-labeled antigen reaction with antibodies directed against both the antigen and the fluorophore. Labeled and unlabeled antigens compete for a limited number of anti-antigen antibodies

Product #

Description

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07985 6-Aminofluorescein BioReagent, suitable for fluorescence, ~95% (HPLC)
10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt for fluorescence, ≥97.0% (HPLC)
75068 9,10-Anthracenediyl-bis(methylene)dimalonic acid BioReagent, suitable for fluorescence, ≥90% (HPCE)
70706 Atto 520 BioReagent, suitable for fluorescence, ≥90.0% (HPCE/HPLC)
77810 Atto 520 NHS ester BioReagent, suitable for fluorescence, ≥80.0% (coupling to amines)
75784 Atto 565 BioReagent, suitable for fluorescence, ≥75% (sum of isomers, HPCE/HPLC)
72464 Atto 565 NHS ester BioReagent, suitable for fluorescence, ≥70.0% (coupling to amines)
70425 Atto 590 BioReagent, suitable for fluorescence, ≥90% (HPCE)
79636 Atto 590 NHS ester BioReagent, suitable for fluorescence, ≥60% (coupling to amines)
78493 Atto 610 BioReagent, suitable for fluorescence, ≥85.0% (HPCE/HPLC)
93259 Atto 610-NHS ester BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
93711 Atto 655 BioReagent, suitable for fluorescence, ≥85% (HPLC)
76245 Atto 655 NHS ester BioReagent, suitable for fluorescence
94875 Atto 680 BioReagent, suitable for fluorescence, ≥90% (HPLC)
75999 Atto 680 NHS ester BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
72755 5-(Bromomethyl)fluorescein BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)
72301 9-(2-Carboxy-2-cyanovinyl)julolidine BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
21953 5(6)-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥85% (sum of isomers, HPCE)
55222 6-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥90% (HPLC)
53048 5-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence, ≥70% (coupling to amines)
88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
25455 4-Chloro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
72335 9-(2,2-Dicyanovinyl)julolidine BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)
46950 Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
55817 Fluorescent red 630 ≥85% (HPLC)
78361 Fluorescent red 630 reactive for fluorescence, ≥80% (coupling to amines)
47614 Fluram BioReagent, suitable for fluorescence, ≥99.0% (UV)
64958 2-Methoxy-2,4-diphenyl-3(2H)-furanone suitable for fluorescence, ≥98.0% (HPLC)
94457 NIR-641 N-succinimidyl ester ≥80% (HPLC), suitable for fluorescence, BioReagent
86186 Sulforhodamine B acid chloride suitable for fluorescence, technical