Protein Stains For Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

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Description

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05497 Albumin blue 580 potassium salt solution for fluorescence, in isopropanol
06880 4-Aminobenzamidine dihydrochloride BioReagent, suitable for fluorescence, ≥99.0% (TLC)
08445 7-Amino-4-methyl-3-coumarinylacetic acid BioReagent, suitable for fluorescence, ~90% (HPLC)
10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt for fluorescence, ≥97.0% (HPLC)
10419 8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate for fluorescence, ≥95.0% (T)
75068 9,10-Anthracenediyl-bis(methylene)dimalonic acid BioReagent, suitable for fluorescence, ≥90% (HPCE)
70706 Atto 520 BioReagent, suitable for fluorescence, ≥90.0% (HPCE/HPLC)
77810 Atto 520 NHS ester BioReagent, suitable for fluorescence, ≥80.0% (coupling to amines)
75784 Atto 565 BioReagent, suitable for fluorescence, ≥75% (sum of isomers, HPCE/HPLC)
72464 Atto 565 NHS ester BioReagent, suitable for fluorescence, ≥70.0% (coupling to amines)
70425 Atto 590 BioReagent, suitable for fluorescence, ≥90% (HPCE)
79636 Atto 590 NHS ester BioReagent, suitable for fluorescence, ≥60% (coupling to amines)
78493 Atto 610 BioReagent, suitable for fluorescence, ≥85.0% (HPCE/HPLC)
93259 Atto 610-NHS ester BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
93711 Atto 655 BioReagent, suitable for fluorescence, ≥85% (HPLC)
76245 Atto 655 NHS ester BioReagent, suitable for fluorescence
94875 Atto 680 BioReagent, suitable for fluorescence, ≥90% (HPLC)
75999 Atto 680 NHS ester BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
12266 N-[4-(2-Benzimidazolyl)phenyl]maleimide BioReagent, suitable for fluorescence, ≥98.0% (HPLC)
52543 [2,6-Bis(2-pyridyl)phenyl-C,N,N′]chloroplatinum(II) Long lifetime reagent for time-resolved and two-photon emission imaging microscopy
72755 5-(Bromomethyl)fluorescein BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)
17623 4-Bromomethyl-7-methoxycoumarin for fluorescence, ≥97.0% (HPLC)
72301 9-(2-Carboxy-2-cyanovinyl)julolidine BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
21953 5(6)-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥85% (sum of isomers, HPCE)
55222 6-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥90% (HPLC)
53048 5-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence, ≥70% (coupling to amines)
88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
30432 Dansylcadaverine BioReagent, suitable for fluorescence, ≥99.0% (HPLC)
39220 Dansyl chloride BioReagent, suitable for fluorescence, ≥99.0% (HPLC)
72335 9-(2,2-Dicyanovinyl)julolidine BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
46950 Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
55817 Fluorescent red 630 ≥85% (HPLC)
78361 Fluorescent red 630 reactive for fluorescence, ≥80% (coupling to amines)
57672 N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid suitable for fluorescence, ≥95% (HPLC)
63184 5-Maleimido-eosin for fluorescence, ≥93% (HPLC)
64958 2-Methoxy-2,4-diphenyl-3(2H)-furanone suitable for fluorescence, ≥98.0% (HPLC)
69899 Monochlorobimane suitable for fluorescence, ≥70.0% (HPCE)
94457 NIR-641 N-succinimidyl ester ≥80% (HPLC), suitable for fluorescence, BioReagent
15167 NIR-797 isothiocyanate suitable for fluorescence, ≥70% (coupling to amines)
17866 Streptavidin−CryptoFluor Crimson suitable for fluorescence