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Functional Group Reactive Labels for Immunoassays

General Features & Benefits
  • Immunological methods are based upon the competitive binding of labeled ligands versus unlabeled ones on highly specific receptor sites
  • Very important tools for clinical and analytical biochemistry and the analysis of drugs, pesticides, hormones and proteins
  • Outstanding specificity and sensitivity, adaptability to automation & an ability to quantitate an almost limitless number of analytes.
  • Competitive (higher selectivity) and non-competitive (higher sensitivity) immunoassay design

Heterogeneous Immunoassays
  • Physical separation of bound from free labeled analyte. Mostly the analyte or antibody is attached to a solid surface
  • Detection can be direct (only a primary antibody is used) or indirect (a secondary antibody is also used)

Homogenous Immunoassays
  • Fluorescence polarization excitation of fluorochromes
  • Double-antibody fluorophore-labeled antigen reaction with antibodies directed against both the antigen and the fluorophore. Labeled and unlabeled antigens compete for a limited number of anti-antigen antibodies

Product #

Description

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07985 6-Aminofluorescein BioReagent, suitable for fluorescence, ~95% (HPLC)
201634 6-Aminofluorescein
21965 5(6)-Carboxy-X-rhodamine BioReagent, suitable for fluorescence
21966 5(6)-Carboxy-X-rhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
21953 5(6)-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥85% (sum of isomers, HPCE)
25455 4-Chloro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
163260 4-Chloro-7-nitrobenzofurazan 98%
46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)
F3651 Fluorescein 5(6)-isothiocyanate ≥90% (HPLC)
46950 Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
L0259 Lucifer Yellow CH dilithium salt fluorescent stain
L0144 Lucifer Yellow CH dipotassium salt powder
68617 10-Methyl-9-(phenoxycarbonyl)acridinium fluorosulfonate ≥90% (HPLC)
87918 Tetramethylrhodamine isothiocyanate mixed isomers suitable for fluorescence, mixture of isomers