Carbohydrate Labels for Chromatography

Advantages of Fluorescent Derivatization:
  • Measured light parameters are positive (light signal is zero in absence of fluorophore)
  • Fluorophoric species are relatively rare in analytical matrices resulting in low background noise levels
  • Detection problems caused by lack of universal detectors for HPLC can be solved partially by UV and fluorescence derivatization techniques
  • High sensitivity
  • Pre-column derivatization offers simplicity and less reagent demand as compared with post-column derivatization
  • Pre- and post-column derivatization techniques are available for both column chromatography and thin layer chromatography, including reversed-phase chromatography (including ion pair chromatography) and ion exchange chromatography (IEC) systems

Our Glyco Profile Labeling Kits are designed for efficient labeling of N-linked, O-linked and glycosylphosphatidylinositol (GPI) anchored glycans using your choice of 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). See our Glycoproteomics Selection Guide for additional details on labeling, detection and quantitation.

Product #

Description

Assay

Fluorescence

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70684 1-(2-Naphthoyl)imidazole ≥95.0% (N)
λex 234 nm; λem 274 nm in H2O
λex 234 nm; λem 374 nm (after derivatization)
438952 1-Naphthaleneacetic anhydride 96%
λex 216 nm; λem 360 nm (polyamin adduct)
64958 2-Methoxy-2,4-diphenyl-3(2H)-furanone ≥98.0% (HPLC)
λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)
66807 4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride ≥99.0% (HPLC)
λex 367 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate)
65877 4-(6-Methyl-2-benzothiazolyl)phenyl isocyanate ≥98.0% (HPLC)
λex 327 nm; λem 382 nm in methylene chloride (after derivatization with hexylamine)
λex 350 nm; λem 374 nm in chlorobenzene
17623 4-Bromomethyl-7-methoxycoumarin ≥97.0% (HPLC)
λex 312 nm; λem 395 nm in methanol
25455 4-Chloro-7-nitrobenzofurazan ≥97.0% (HPLC)
λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)
47140 4-Fluoro-7-nitrobenzofurazan ≥98.0% (HPLC)
λex 470 nm; λem 528 nm in ethanol
λex 470 nm; λem 550 nm (Amino acid adducts)
64785 4-Methoxybenzamidine ≥96.0% (NT)
λex 393 nm; λem 478 nm (after derivatization with glucose)
λex 393 nm; λem 478 nm(lit.)
07985 6-Aminofluorescein ~95% (HPLC)
λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0
36798 7-(Diethylamino)coumarin-3-carbohydrazide ≥95% (HPCE)
λex 450 nm; λem 468 nm in methanol
31755 7-(Diethylamino)coumarin-3-carbonyl azide ≥95% (HPLC)
λex 390 nm; λem 478 nm in methanol
46640 7-Fluorobenzofurazan-4-sulfonic acid ammonium salt ≥98.5% (HPLC)
λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)
08658 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt ≥90% (CE)
λex 356 nm; λem 512 nm in 0.1 M phosphate pH 7.0
09341 8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt ≥96.0% (HPCE)
λex 420 nm; λem 500 nm in 0.1 M Tris pH 7.4 (quenching with Vitamin B1)
λex 488 nm; λem 520 nm(lit.)
25102 9-(Chloromethyl)anthracene ≥97.0% (AT)
λex 356 nm; λem 412 nm (after derivatisation with sodium acetate)
10609 9-Anthracenecarbonyl cyanide ≥98.5% (GC)
λex 361 nm; λem 451 nm (after derivatization with ethanol)
λex 361 nm; λem 460 nm in methanol
75707 Bis(2,4,6-trichlorophenyl) oxalate ≥99.0% (AT)
 
39220 Dansyl chloride ≥99.0% (HPLC)
λex 337 nm; λem 492 nm in chloroform (after derivatization with hexylamine)
30434 Dansylhydrazine ≥90% (HPLC)
λex 340 nm; λem 525 nm in ethanol
42815 Diphenylborinic anhydride ≥95.0% (T)
 
46985 Fluorescein-5-thiosemicarbazide ~80% (HPCE)
λex 492 nm; λem 516 nm in 0.1 M Tris pH 9.0
47614 Fluram ≥99.0% (UV)
λex 234 nm
λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
23184 Fmoc chloride ≥99.0% (HPLC)
 
PP0530 GlycoProfile 2-AA Labeling Kit    
PP0520 GlycoProfile 2-AB Labeling Kit    
M8010 N,N′-Dimethyl-9,9′-biacridinium dinitrate   λex 319 nm; λem 384 nm (pH 9.0)
λex 360 nm; λem 449 nm (Reaction product)
λex 368 nm; λem 505 nm in H2O
82656 N-(1-Pyrenyl)maleimide ≥99.0% (HPLC)
 
57672 N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid ≥95% (HPLC)
λex 336 nm; λem 490 nm (pH 8.0)
λex 337 nm; λem 498 nm (after derivatization with 2-mercaptoethanol)
79760 Phthaldialdehyde ≥99.0% (HPLC)
λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)
78410 o-Phenylenediamine ≥99.0% (NT)