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Carbohydrate Labels for Chromatography



Advantages of Fluorescent Derivatization:
  • Measured light parameters are positive (light signal is zero in absence of fluorophore)
  • Fluorophoric species are relatively rare in analytical matrices resulting in low background noise levels
  • Detection problems caused by lack of universal detectors for HPLC can be solved partially by UV and fluorescence derivatization techniques
  • High sensitivity
  • Pre-column derivatization offers simplicity and less reagent demand as compared with post-column derivatization
  • Pre- and post-column derivatization techniques are available for both column chromatography and thin layer chromatography, including reversed-phase chromatography (including ion pair chromatography) and ion exchange chromatography (IEC) systems

Our Glyco Profile Labeling Kits are designed for efficient labeling of N-linked, O-linked and glycosylphosphatidylinositol (GPI) anchored glycans using your choice of 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). See our Glycoproteomics Selection Guide for additional details on labeling, detection and quantitation.

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706841-(2-Naphthoyl)imidazole ≥95.0% (N)
λex 234 nm; λem 374 nm (after derivatization)
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709301-Naphthaleneacetic anhydride ≥97.0% (T)
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4389521-Naphthaleneacetic anhydride 96%
λex 216 nm; λem 360 nm (polyamin adduct)
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649582-Methoxy-2,4-diphenyl-3(2H)-furanone ≥98.0% (HPLC)
λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)
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668074,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride ≥99.0% (HPLC)
λex 367 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate)
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658774-(6-Methyl-2-benzothiazolyl)phenyl isocyanate ≥98.0% (HPLC)
λex 327 nm; λem 382 nm in methylene chloride (after derivatization with hexylamine)
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254554-Chloro-7-nitrobenzofurazan ≥99.0% (HPLC)
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)
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471404-Fluoro-7-nitrobenzofurazan ≥98.0% (HPLC)
λex 470 nm; λem 550 nm
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538924-Hydrazino-7-nitrobenzofurazan ≥95.0% (TLC)
λex 445 nm; λem 522 nm in chloroform (after derivatization with formaldehyde)
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647854-Methoxybenzamidine ≥97.0% (NT)
λex 393 nm; λem 478 nm (after derivatization with glucose)
λex 393 nm; λem 478 nm(lit.)
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079856-Aminofluorescein ~95% (HPLC)
λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0
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367987-(Diethylamino)coumarin-3-carbohydrazide ≥95% (HPCE)
λex 450 nm; λem 468 nm in methanol
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317557-(Diethylamino)coumarin-3-carbonyl azide ≥95% (HPLC)
λex 390 nm; λem 478 nm in methanol
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084407-Amino-4-methylcoumarin ≥96.0% (UV)
λex 351 nm; λem 430 nm in methanol
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466407-Fluorobenzofurazan-4-sulfonic acid ammonium salt ≥98.5% (HPLC)
λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)
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086588-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt ≥90% (CE)
λex 356 nm; λem 512 nm in 0.1 M phosphate pH 7.0
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093418-Aminopyrene-1,3,6-trisulfonic acid trisodium salt ≥96.0% (HPCE)
λex 488 nm; λem 520 nm(lit.)
λex 420 nm; λem 500 nm in 0.1 M Tris pH 7.4 (quenching with Vitamin B1)
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251029-(Chloromethyl)anthracene ≥97.0% (AT)
λem 412 nm (after derivatisation with sodium acetate)
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106099-Anthracenecarbonyl cyanide ≥98.5% (GC)
λex 361 nm; λem 451 nm (after derivatization with ethanol)
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106039-Anthracenecarboxaldehyde ≥97.0% (HPLC)
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10678Anthranilic acid ≥99.0% (T)
λex 315 nm; λem 400 nm(lit.)
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75707Bis(2,4,6-trichlorophenyl) oxalate ≥99.0% (AT)
λem 430 nm
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75705Bis(2,4-dinitrophenyl) oxalate ≥97.0% (TLC)
λem 430 nm
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21795Carbazole-9-carbonyl chloride ≥98.0% (GC)
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39220Dansyl chloride ≥99.0% (HPLC)
λex 337 nm; λem 492 nm in chloroform (after derivatization with hexylamine)
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39223Dansyl fluoride ≥99.0% (HPLC)
λex 360 nm; λem 580 nm in methanol
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30434Dansylhydrazine ≥95.0% (HPLC)
λex 340 nm; λem 520 nm in ethanol
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42815Diphenylborinic anhydride ≥95.0% (T)
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46985Fluorescein-5-thiosemicarbazide ~80% (HPCE)
λex 492 nm; λem 516 nm in 0.1 M Tris pH 9.0
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47614Fluram® ≥99.0% (UV)
λex 390 nm; λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
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23184Fmoc chloride ≥99.0% (HPLC)
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PP0530GlycoProfile 2-AA Labeling Kit pricing
PP0520GlycoProfile 2-AB Labeling Kit pricing
58980Isoniazid ≥99.0% (NT)
λex 433 nm; λem 500 nm in methanol (after derivatization with progesterone and as complex with Al3+)
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63340Malonamide ≥98.0% (N)
λex 383 nm; λem 425 nm(lit.)
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M8010N,N′-Dimethyl-9,9′-biacridinium dinitrate pricing
82656N-(1-Pyrenyl)maleimide ≥99.0% (HPLC)
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39145N-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide ≥99.0% (HPLC)
λex 398 nm; λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)
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57672N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid ≥95% (HPLC)
λex 337 nm; λem 498 nm (after derivatization with 2-mercaptoethanol)
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12535NIR-664-iodoacetamide ≥90% (HPLC)
λex 664 nm; λem 689 nm in methanol
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79760Phthaldialdehyde ≥99.0% (HPLC)
λex 340 nm; λem 450 nm in reaction buffer; glycine
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79765Phthaldialdehyde ≥97.0% (HPLC)
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78411o-Phenylenediamine pricing
78410o-Phenylenediamine ≥99.0% (NT)
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78441o-Phenylenediamine dihydrochloride pricing
78446o-Phenylenediamine dihydrochloride pricing
53897trans-4′-Hydrazino-2-stilbazole dihydrochloride ≥85% (AT)
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