Protein Stains for Microscopy

Microscopy offers great potential for qualitative and quantitative studies on the structure and function of cells and is uniquely suitable for probing living cells. The specimen is illuminated by light of a certain range of wavelengths, which may not necessarily be monochromatic, while viewing emitted (fluorescence) light of longer wavelengths. New methods, like confocal microscopy, provide better resolution (~1.4 improvement) and enable the measurement of various parameters. A point source and a point detector, both achieved by a pinhole are used, while light from other than the focal plane is defocused at the confocal aperture and thus detected only weakly.
Schematic diagram of a confocal microscope.

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01673 Acriflavine BioReagent, suitable for fluorescence, ≥95.0% (AT)
    • 08445 7-Amino-4-methyl-3-coumarinylacetic acid BioReagent, suitable for fluorescence, ~90% (HPLC)
    • 10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt for fluorescence, ≥97.0% (HPLC)
    • 10419 8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate for fluorescence, ≥95.0% (T)
    • 75068 9,10-Anthracenediyl-bis(methylene)dimalonic acid BioReagent, suitable for fluorescence, ≥90% (HPCE)
    • 70706 Atto 520 BioReagent, suitable for fluorescence, ≥90.0% (HPCE/HPLC)
    • 77810 Atto 520 NHS ester BioReagent, suitable for fluorescence, ≥80.0% (coupling to amines)
    • 75784 Atto 565 BioReagent, suitable for fluorescence, ≥75% (sum of isomers, HPCE/HPLC)
    • 72464 Atto 565 NHS ester BioReagent, suitable for fluorescence, ≥70.0% (coupling to amines)
    • 70425 Atto 590 BioReagent, suitable for fluorescence, ≥90% (HPCE)
    • 79636 Atto 590 NHS ester BioReagent, suitable for fluorescence, ≥60% (coupling to amines)
    • 78493 Atto 610 BioReagent, suitable for fluorescence, ≥85.0% (HPCE/HPLC)
    • 93259 Atto 610-NHS ester BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
    • 93711 Atto 655 BioReagent, suitable for fluorescence, ≥85% (HPLC)
    • 76245 Atto 655 NHS ester BioReagent, suitable for fluorescence
    • 94875 Atto 680 BioReagent, suitable for fluorescence, ≥90% (HPLC)
    • 75999 Atto 680 NHS ester BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
    • 72755 5-(Bromomethyl)fluorescein BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)
    • 72301 9-(2-Carboxy-2-cyanovinyl)julolidine BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
    • 21878 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)
    • 21953 5(6)-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥85% (sum of isomers, HPCE)
    • 55222 6-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥90% (HPLC)
    • 53048 5-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
    • 21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence, ≥70% (coupling to amines)
    • 88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence
    • 72335 9-(2,2-Dicyanovinyl)julolidine BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
    • 40468 Fluorescent orange 548 ≥80% (TLC)
    • 55817 Fluorescent red 630 ≥85% (HPLC)
    • 78361 Fluorescent red 630 reactive for fluorescence, ≥80% (coupling to amines)
    • 62164 Fluorescent red 646 reactive suitable for fluorescence
    • 94457 NIR-641 N-succinimidyl ester ≥80% (HPLC), suitable for fluorescence, BioReagent
    • 15167 NIR-797 isothiocyanate suitable for fluorescence, ≥70% (coupling to amines)