General Features & Benefits- Measured light parameters are positive (light signal is zero in absence of fluorophore)
- Fluorophoric species relatively rare in analytical matrices, low background noise levels are obtained
- Detection problems caused by lack of universal detectors for HPLC can be solved partially, by UV and fluorescence derivatization techniques
- High sensitivity
- Pre-column derivatization offers simplicity and less reagent demand as compared with post-column derivatization
Column Chromatography- Pre- and post-column derivatization techniques
- Reversed-phase chromatography (including ion pair chromatography) frequently applied
- Ion exchange chromatography (IEC) systems
Thin layer ChromatographyPre- and post-separation derivatization techniques
