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Antibody Explorer
Affinity Chromatography
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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in an exclusive partnership with Sigma-Aldrich. Each month, 2 new protocols will be featured, each with a list of products available from Sigma-Aldrich.
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View the entire protocol onBioSupplyNet.com.
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Selected Protocol from "Molecular Cloning: A Laboratory Manual," Third Edition, Joseph Sambrook, David W. Russell.
Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography
Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas
ABSTRACT
This protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of immunoglobulins through a column containing immobilized bacterial proteins.
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Buy this book, M8265 |
| Products Available for this Protocol |
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Sodium borate (pH 8.0) |
B9876 |
Sodium tetraborate decahydrate, ReagentPlus®, 99.5–105.0% |
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| NaCl |
S3014 |
Sodium chloride, for molecular biology, ≥98% (titration) |
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| Pancreatic DNase I |
D4527 |
Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein ~90 %, ≥2,000 units/mg protein |
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| Tris-Cl (pH 7.5) |
T5941 |
Trizma® hydrochloride, for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration) |
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| MgCl2 |
M4880 |
Magnesium chloride, insect cell culture tested |
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| NaOH |
S8045 |
Sodium hydroxide, SigmaUltra, ≥98%, pellets (anhydrous) |
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| TBS |
T5912 |
Tris Buffered Saline, 10X, solution |
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| Sodium azide |
S8032 |
Sodium azide, SigmaUltra |
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| Triton X-100 |
234729 |
Triton® X-100 |
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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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