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Life Science > Cell Biology > Antibodies > Antibodies Applications Center > Protocols > Affinity Chromatography
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Affinity Chromatography | ||
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Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. |
View the entire protocol on BioSupplyNet.com.
Selected Protocol from "Molecular Cloning: A Laboratory Manual," Third Edition, Joseph Sambrook, David W. Russell.
Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography
Joseph Sambrook – Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell – University of Texas Southwestern Medical Center, Dallas
ABSTRACT
This protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of immunoglobulins through a column containing immobilized bacterial proteins.
Products Available for this Protocol
| Protocol Material | Product No. | Description | Add to Cart |
| B9876 | Sodium tetraborate decahydrate, ReagentPlus®, 99.5–105.0% |
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| NaCl | S3014 | Sodium chloride, for molecular biology, ≥98% (titration) |
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| Pancreatic DNase I | D4527 | Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein ~90 %, ≥2,000 units/mg protein |
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| Tris-Cl (pH 7.5) | T5941 | Trizma® hydrochloride, for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration) |
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| MgCl2 | M4880 | Magnesium chloride, insect cell culture tested |
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| NaOH | S8045 | Sodium hydroxide, SigmaUltra, ≥98%, pellets (anhydrous) |
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| TBS | T5912 | Tris Buffered Saline, 10X, solution |
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| Sodium azide | S8032 | Sodium azide, SigmaUltra |
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| Triton X-100 | 234729 | Triton® X-100 |
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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.