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Antibody Explorer
Histological Preparation
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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in a partnership with Sigma-Aldrich. A list of products is available to use in each protocol.
View the protocol on BioSupplyNet.com.
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View the protocol "Histological Preparation of Embryonic and Adult Zebrafish Eyes" at BioSupplynet.com
| Richard J. Nuckels1 and Jeffrey M. Gross1,2,3 |
1 Section of Molecular Cell and Developmental Biology; University of Texas at Austin, Austin, Texas 78712, USA
2 Institute of Cell and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
3 Institute for Neuroscience; University of Texas at Austin, Austin, Texas 78712, USA
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ABSTRACT
This protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapted for use on other zebrafish tissues.
RELATED INFORMATION
These methods have been employed in studies on morphological development of the zebrafish retina (Branchek and Bremiller 1984, Schmitt and Dowling 1999).
| Products Available for this Protocol |
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Phosphate buffered saline (PBS) |
P5368 |
Phosphate buffered saline, pH 7.4, Biotechnology Performance Certified |
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| DPX mountant |
44581 |
DPX Mountant for histology |
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| Epon 812 |
45345 |
Epoxy embedding medium |
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| 2-Dodecenylsuccinic acid anhydride (DDSA) |
44161 |
Dodecenylsuccinic anhydride, technical |
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| DMP-30 |
374555 |
2,4,6-Tris(dimethylaminomethyl)phenol, technical grade, 70% |
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| Ethanol |
E7148 |
Ethanol, for molecular biology, 190 proof |
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| Paraformaldehyde (PFA) |
P6148 |
Paraformaldehyde, reagent grade, crystalline |
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| Sucrose |
S1888 |
Sucrose, cell culture tested, insect cell culture tested, ≥99.5% |
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| Glutaraldehyde |
G5882 |
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use |
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| Azure B |
861057 |
Azure B, certified by the Biological Stain Commission, Dye content 89% |
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| Methylene blue |
M9140 |
Methylene blue, certified by the Biological Stain Commission |
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| Osmium tetroxide |
201030 |
Osmium tetroxide, ReagentPlus®, 99.8% |
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| Propylene oxide |
110205 |
(±)-Propylene oxide, ReagentPlus®, 99% |
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| Tricaine |
A5040 |
Ethyl 3-aminobenzoate methanesulfonate salt |
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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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