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Antibody Explorer
Yeast Chip Assay
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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in an exclusive partnership with Sigma-Aldrich. Each month, 2 new protocols will be featured, each with a list of products available from Sigma-Aldrich.
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Yeast Chromatin Immunoprecipitation (ChIP) Assay
William P. Tansey
ABSTRACT
This protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of this assay.
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Products Available for this Protocol
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| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Buffers, Solutions, and Reagents |
| Antibody of choice |
Visit the Antibody Explorer for all your antibody needs. |
| NaCl |
S3014 |
Sodium chloride, for molecular biology |
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| NP-40 |
NP40S |
Tergitol® solution, Type NP-40, 70% in H2O |
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| Deoxycholate (DOC) |
D6875 |
21-Hydroxyprogesterone |
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| SDS (10%) |
L4390 |
Sodium dodecyl sulfate, for molecular biology |
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| Tris-Cl |
T5941 |
Trizma® hydrochloride, for molecular biology, Biotechnology Performance Certified |
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| Aprotinin |
A6103 |
Aprotinin from bovine lung, recombinant, expressed in Nicotiana |
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| Pepstatin |
P5318 |
Pepstatin A |
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| Leupeptin |
L9783 |
Leupeptin hydrochloride |
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| Pefabloc |
76307 |
Pefabloc® SC |
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| LiCl |
L9650 |
Lithium chloride, for molecular biology |
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| Ethanol |
E7023 |
Ethanol, 200 proof (absolute), for molecular biology |
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| Glycine |
G8790 |
Glycine, meets EP, JP, USP testing specifications, cell culture tested |
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| Glycogen |
G1767 |
Glycogen from Mytilus edulis (Blue mussel) |
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| NaOAc |
S5636 |
Sodium acetate, cell culture tested, insect cell culture tested |
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| Paraformaldehyde (PFA) |
158127 |
Paraformaldehyde, powder, 95% |
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| Phenol:chloroform |
P1944 |
Phenol:Chloroform 5:1, for molecular biology |
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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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