Actin Antibodies

Sigma Life Science offers a portfolio of polyclonal and monoclonal Actin antibodies. Select appropriate Actin antibodies for your research by form, gene symbol, species reactivity or application. Each Actin antibody is covered by our Bioguarantee so that you can evaluate our Actin antibodies with complete peace of mind.

The Actin Gene Family
Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Actin is one of the most conserved eukaryotic proteins with at least six isoforms. Four of the isoforms represent the differentiation markers of muscle tissues and two are found in almost all cells. There are three a-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Actin isoforms show greater than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. The NH2-terminal region of actin appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin.
Note: Historically, due to its ubiquitous nature, β-Actin was used as an internal standard for Western blotting. More recently its levels have been shown to fluctuate following different treatments. Therefore, GAPDH is becoming more popular as a housekeeping gene.

Product #

Description

Clone

Form

Gene Symbol (ID)

Species Reactivity

Application

Add to Cart

A2066 Anti-Actin antibody produced in rabbit polyclonal
affinity isolated antibody
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
amoeba, wide range, chicken, slime mold, vertebrates, human
IF, IHC (p), WB
A5060 Anti-Actin (20-33) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
wide range (animal), invertebrates
ARR, IF, IHC (p), WB
A3854 Monoclonal Anti-β-Actin−Peroxidase antibody produced in mouse AC-15, monoclonal
purified immunoglobulin
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine
WB
A3853 Monoclonal Anti-Actin antibody produced in mouse AC-40, monoclonal
purified immunoglobulin
  goat, viper, Xenopus, bovine, guinea pig, snail, canine, hamster, rat, chicken, mouse, human, sheep, pig, rabbit, carp
ARR, ELISA (i), ICC, IHC, WB
A4700 Monoclonal Anti-Actin antibody produced in mouse AC-40, monoclonal
ascites fluid
  goat, viper, Xenopus, bovine, guinea pig, snail, canine, hamster, rat, chicken, mouse, human, sheep, pig, rabbit, carp
ELISA (i), IF, IHC (f), WB
A2228 Monoclonal Anti-β-Actin antibody produced in mouse AC-74, monoclonal
purified immunoglobulin
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
canine, guinea pig, rabbit, bovine, chicken, rat, sheep, wide range, carp, Drosophila, Hirudo medicinalis, cat, human, mouse, pig
ARR, ICC, IHC, WB
A5316 Monoclonal Anti-β-Actin antibody produced in mouse AC-74, monoclonal
ascites fluid
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
canine, guinea pig, rabbit, bovine, chicken, rat, sheep, wide range, carp, Drosophila, Hirudo medicinalis, cat, human, mouse, pig
ELISA (i), IF, IHC (p), WB
A5441 Monoclonal Anti-β-Actin antibody produced in mouse AC-15, monoclonal
ascites fluid
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine
ELISA (i), IF, IHC (p), WB
A8481 Monoclonal Anti-γ-Actin antibody produced in mouse 2-2.1.14.17, monoclonal
purified immunoglobulin
bovine ... ACTG1(404122)
canine ... LOC475123(475123)
chicken ... RCJMB04_1h13(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)
canine, human, bovine, mouse, hamster, chicken
ELISA (i), IHC, WB
A2103 Anti-Actin, N-terminal antibody produced in rabbit polyclonal
affinity isolated antibody
human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72)
mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468)
rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365)
mouse, frog, human, chicken, rat
ICC, IF, IHC (p), WB
A1978 Anti-β-Actin antibody, Mouse monoclonal AC-15, monoclonal
purified immunoglobulin
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine
ARR, ICC, IF, IHC (f), WB
A2172 Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse 5C5, monoclonal
ascites fluid
human ... ACTA1(58), ACTC1(70)
mouse ... Rwdd2a(69519)
rat ... Acta1(29437), Actc1(29275)
sheep, snake, human, rabbit, guinea pig, rat, frog, bovine, carp
ELISA (i), IHC (f), IHC (p), WB
A5228 Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal 1A4, monoclonal
purified immunoglobulin
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine
ARR, ELISA (i), ICC, IF, IHC (p), WB