ChIP Validated Antibodies

Successful chromatin immunoprecipitation (ChIP) requires an effective, specific, and high quality antibody. Not all antibodies work in ChIP as the antigen epitope may be blocked or altered once cross-linked. Sigma′s Imprint brand of antibodies are validated in ChIP application and lot tested each time for successful chromatin immunoprecipitation experiment.

Chromatin immunoprecipitation (ChIP) is a powerful tool for identifying proteins, including histone proteins and non-histone proteins, associated with specific regions of the genome by using specific antibodies that recognize a specific protein or a specific modification of a protein. The ChIP protocol is based on the principle that formaldehyde reacts with primary amines located on amino acids and the bases on DNA or RNA molecules, forming a covalent crosslink between the specific protein to the DNA on which they are situated. Once the DNA binding proteins are crosslinked, the cells are lysed and the crude cell extracts are sonicated to shear the DNA to a smaller size. The protein:DNA complex is immunoprecipitated using an antibody against the protein of interest. The DNA protein cross-links are reversed by heating and the proteins removed by treatment with proteinase K. The DNA portion of the complex is then purified and identified by PCR using specific primers to the suspected binding region.

Product #

Description

Form

Gene Symbol (ID)

Application

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17-10032 ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonal      
17-10034 ChIPAb+ EED - ChIP Validated Antibody and Primer Set      
17-10044 ChIPAb+ CTCF - ChIP Validated Antibody and Primer Set      
17-10047 ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal      
17-10048 ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set      
17-10054 ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set      
17-10057 ChIPAb+ SMRT - ChIP Validated Antibody and Primer Set      
17-10060 ChIPAb+ NFκB p65 (RelA) - ChIP Validated Antibody and Primer Set      
17-10099 ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set      
17-10101 ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set      
17-10135 ChIPAb+ CBX8 - ChIP Validated Antibody and Primer Set      
17-10136 ChIPAb+ Dimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set      
17-10237 ChIPAb+™ HDAC2 - ChIP Validated Antibody and Primer Set      
17-10245 ChIPAb+™ Histone H3.3 - ChIP Validated Antibody and Primer Set      
17-10246 ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set      
17-10250 ChIPAb+™ Dimethyl-Histone H4 (Arg3) Symmetric - ChIP Validated Antibody and Primer Set      
17-10252 ChIPAb+™ LSF - ChIP Validated Antibody and Primer Set      
17-10254 ChIPAb+™ Histone H3 (CT) - ChIP Validated Antibody and Primer Set      
17-10256 ChIPAb+™ SOX-2 - ChIP Validated Antibody and Primer Set      
17-10258 ChIPAb+™ FOXA2 - ChIP Validated Antibody and Primer Set      
17-10259 ChIPAb+ Acetyl-Histone H3 (Lys56) - ChIP Validated Antibody and Primer Set      
17-10260 ChIPAb+™ N-CoR Antibody      
17-10263 ChIPAb+™ JMJD6 - ChIP Validated Antibody and Primer Set      
17-10264 ChIPAb+™ JHDM1B Antibody      
17-10267 ChIPAb+™ FOXA1 Antibody      
17-10456 ChIPAb+ REST - ChIP Validated Antibody and Primer Set      
17-10489 ChIPAb+ Androgen Receptor- ChIP Validated Antibody and Primer Set      
17-10491 ChIPAb+ MeCP2 - ChIP Validated Antibody and Primer Set      
17-10493 ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set      
17-10498 ChIPAb+ Monomethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set      
17-10530 ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set      
17-10531 ChIPAb+ LSD1 (KDM1A) Antibody - ChIP Validated Antibody and Primer Set      
17-601 ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set      
17-614 ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal      
17-615 ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set      
17-620 ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set      
17-622 ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set      
17-625 ChIPAb+ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set      
17-650 ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set      
17-655 ChIPAb+ Nanog Antibody - ChIP Validated Antibody and Primer Set      
17-662 ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set      
17-672 ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set      
17-678 ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set      
17-683 ChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set      
17-685 ChIPAb+ Phospho-Histone H3 (Ser10) - ChIP Validated Antibody and Primer Set      
SAB4800001 Imprint® Monoclonal Anti-5-methylcytosine (33D3) antibody produced in mouse affinity isolated antibody
  ELISA (i), FACS, IF (d), MeDIP
SAB4800002 Imprint® Monoclonal Anti-5-hydroxymethylcytosine antibody produced in rat affinity isolated antibody
  ELISA (i), MeDIP
SAB4800003 Imprint® Anti-H3K4me3, (N-terminal) antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, WB
SAB4800004 Imprint® Anti-H3K9me3 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800006 Imprint® Anti-H3K36me3 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800007 Imprint® Anti-H3K9/14ac antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800010 Imprint® Anti-H3K4me1, (N-terminal) antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800011 Imprint® Monoclonal Anti-TBP, (N-terminal) antibody produced in mouse purified antibody
human ... TBP(6908)
ChIP, IHC (p)
SAB4800012 Imprint® Anti-MeCP2 antibody produced in rabbit affinity isolated antibody
human ... MECP2(4204)
ChIP, ELISA (i), WB
SAB4800013 Imprint® Anti-TIP5 antibody produced in rabbit whole antiserum
human ... BAZ2A(11176)
ChIP, WB
SAB4800014 Imprint® Anti-HDAC1, (C-terminal) antibody produced in rabbit affinity isolated antibody
human ... HDAC1(3065)
ChIP, ELISA (i), WB
SAB4800016 Imprint® Anti-H3K9me1 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800017 Imprint® Anti-H4K20me3 antibody produced in rabbit affinity isolated antibody
human ... H4F3(3023)
ChIP, ELISA (i), IF (d), WB
SAB4800018 Imprint® Monoclonal Anti-H3K9me3 antibody produced in mouse affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, WB
SAB4800019 Imprint® Monoclonal Anti-HDAC1, (C-terminal) antibody produced in mouse affinity isolated antibody
human ... HDAC1(3065)
ChIP, WB
SAB4800021 Imprint® Anti-H3K36me1 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800023 Imprint® Anti-H3S10p antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), IF (d), WB
SAB4800024 Imprint® Anti-H4Pan antibody produced in rabbit affinity isolated antibody
human ... H4F3(3023)
ChIP, ELISA (i)
SAB4800026 Imprint® Anti-H3K4me3, (C-terminal) antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800029 Imprint® Anti-H3K79me2 antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800030 Imprint® Monoclonal Anti-H3K9me3 antibody produced in rabbit culture supernatant
human ... H3F3A(3021), H3F3B(3021)
ChIP, WB
SAB4800032 Imprint® Monoclonal Anti-Ty1 antibody produced in mouse purified antibody
  ChIP, WB
SAB4800033 Imprint® Anti-H3K27me3S28p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), IF (d), IP, WB
SAB4800035 Imprint® Anti-H4K8ac antibody produced in rabbit whole antiserum
human ... H4F3(3023)
ChIP, ELISA (i), WB
SAB4800036 Imprint® Anti-H3S10p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), IF (d), IP, WB
SAB4800037 Imprint® Anti-H3K36me2 antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800038 Imprint® Anti-H3R17me2 antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800040 Imprint® Monoclonal Anti-ERa antibody produced in mouse purified antibody
human ... ESR1(2099)
ChIP, IHC (p), WB
SAB4800041 Imprint® Monoclonal Anti-GR antibody produced in mouse purified antibody
human ... NR3C1(2908)
ChIP, ELISA (i), IHC (p), IP, WB
SAB4800042 Imprint® Anti-H2Bpan antibody produced in rabbit affinity isolated antibody
human ... H2BFM(286436)
ChIP, ELISA (i)
SAB4800043 Imprint® Anti-H3K79me1 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800044 Imprint® Anti-H3K9acS10p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800045 Imprint® Anti-H3K9me3S10p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800046 Imprint® Anti-H4K8ac antibody produced in rabbit purified antibody
human ... H4F3(3023)
ChIP, ELISA (i), WB
SAB4800048 Imprint® Monoclonal Anti-Ago antibody produced in mouse affinity isolated antibody
human ... EIF2C2(27161)
IF (d), IHC (p), RIP, WB
SAB4800049 Imprint® Monoclonal Anti-5-hydroxymethylcytosine antibody produced in mouse purified antibody
  ELISA (i), MeDIP
SAB5100001 Imprint® ChIP-Validated Monoclonal Anti-p53 (DO-1) antibody produced in mouse purified immunoglobulin
human ... TP53(7157)
ChIP, WB
SAB5100002 Imprint® ChIP-Validated Monoclonal Anti-p53 (DO-7) antibody produced in mouse purified immunoglobulin
human ... TP53(7157)
ChIP, WB
SAB5100003 Imprint® ChIP-Validated Monoclonal Anti-p53 (PAb1801) antibody produced in mouse purified immunoglobulin
human ... TP53(7157)
ChIP, WB
SAB5100004 Imprint® ChIP-Validated Monoclonal Anti-p53 (BP53-12) antibody produced in mouse ascites fluid
  ChIP, WB
SAB5100005 Imprint® ChIP-Validated Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse purified immunoglobulin
human ... POU5F1(5460)
ChIP, WB
SAB5100006 Imprint® ANTI-POU5F1 (OCT4) antibody produced in rabbit affinity isolated antibody
human ... POU5F1(5460)
ChIP, WB
SAB5100007 Imprint®ChIP-Validated Anti-Acetyl-Histone H3 (Ac-Lys9) (H3K9ac) antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
ChIP
SAB5100008 Imprint® ChIP-Validated Anti-methyl-Histone H3 (Me-Lys9) (H3K9me1) affinity isolated antibody
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
ChIP