Peroxidase Labeled Antibodies

The ability of horseradish peroxidase to oxidize a variety of substrates to yield colored, fluorescent, or luminescent end products makes peroxidase labeled antibodies useful for a variety of applications. The Sigma-Aldrich portfolio of peroxidase labeled antibodies is ideal for use in applications such as ELISA, dot blot, immunohistochemistry, and Western blot, both colorimetric and chemiluminescent. Sigma-Aldrich offers peroxidase labeled secondary antibodies targeting primary antibodies from an extensive array of species including mouse, rabbit, goat, sheep, human, rat, guinea pig, bovine, chicken, horse, dog, pig, and monkey. Peroxidase labeled antibodies are available to specifically detect the whole antibody molecule, the Fc or Fab region only, or specific chains including specific heavy chains (γ-chain, μ-chain, or α-chain) and specific light chains (kappa or lambda).

Antisera are developed in rabbit, goat, sheep, mouse, or donkey using purified immunoglobulin as the immunogen. The isolated antibody is then conjugated to peroxidase by chemical crosslinking of the proteins. Isolation by immunospecific purification prior to conjugation ensures the peroxidase labeled antibody will be essentially free of other serum proteins. Additionally, peroxidase labeled F(ab′)2 fragments are available for highly specific results. F(ab′)2fragments contain only the antigen-binding site of the antibody with the Fc fragment removed. The use of F(ab′)2 fragments helps to eliminate unwanted background reactivity that may be caused by endogenous Fc receptors binding to the Fc region of the peroxidase labeled antibody.

Each peroxidase labeled antibody is covered by our Bioguarantee so that you can evaluate our antibodies with complete peace of mind.