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Antibodies to Histone Modifications

In eukaryotic cells DNA is associated with histones and other proteins to form chromatin. The basic unit of chromatin is the nucleosome consisting of 140 bp of DNA wrapped around an octameric core of the four conserved histones H2A, H2B, H3 and H4.

The relatively unstructured and highly charged N-terminal tail domains of histones, are central to the processes that modulate chromatin structure. A diverse and elaborate array of post-translational modifications that include acetylation, phosphorylation, methylation, ubiquitination and ADP-ribosylation, occur on the N-terminal tail domains of histones. Acetylation of lysine residues within these N-terminal domains by histone acetyl-transferases (HATs), is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation of H3, referred to as the nucleosomal response, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli. Phosphorylation of histone H3 on Ser10 is highly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at this site is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. PKA, Rsk-2 and Msk-1 are necessary for the histone H3 phosphorylation. Activation of both ERK and p38 MAP Kinase pathways by mitogenic stimuli, have been suggested to modulate the phosphorylation of histone H3.

Product #

Description

Clone

Form

Gene Symbol (ID)

Species Reactivity

Application

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H0913 Anti-acetyl-Histone H3 (Ac-Lys9) antibody, Mouse monoclonal AH3-120, monoclonal
purified immunoglobulin
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
human, mouse, bovine, chicken, frog, rat, Drosophila, Caenorhabditis elegans
ARR, ELISA (i), ICC, WB
H9164 Anti-acetyl-Histone H4 (Ac-Lys16) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST4H4(121504)
human
WB
SAB4200045 Monoclonal Anti-Dimethyl-Histone H3 (diMe-Lys27) antibody produced in rat K27 8H2, monoclonal
purified immunoglobulin
  Drosophila, mouse, human, rat, hamster, bovine
ICC, WB
H7789 Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST1H1E(3008)
human
WB
H8289 Anti-dimethyl-Histone H1.4 (diMe-Lys26) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST1H1E(3008)
human
WB
H8164 Anti-dimethyl, phospho-Histone H1.4 (diMe-Lys26, pSer27) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST1H1E(3008)
human
WB
H7665 Anti-Histone H1.4 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST1H1E(3008)
human
WB
H5912 Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... H2AFX(3014)
mouse ... H2afx(15270)
mouse, human
ARR, WB
H0164 Anti-Histone H3 antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
mouse, plant, human, rat, Drosophila, chicken, Xenopus
IF, WB
H9286 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
Drosophila, human, bovine, chicken, frog, Caenorhabditis elegans, rat, mouse
ARR, IF, WB
H0788 Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse APH3-64, monoclonal
purified immunoglobulin
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
frog, rat, Drosophila, Caenorhabditis elegans, human, mouse, bovine, chicken
ARR, ELISA (i), IP, WB
H9161 Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
bovine, frog, human, mouse, Drosophila, Caenorhabditis elegans, rat, chicken
ARR, IF, WB
D5692 Anti-dimethyl-Histone H3 (diMe-Lys4) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
Caenorhabditis elegans, Arabidopsis thaliana, human, Drosophila, bovine, frog, rat, chicken, mouse
ARR, ChIP, WB
D5567 Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
Arabidopsis thaliana, Drosophila, Caenorhabditis elegans, human, bovine, chicken, frog, rat, mouse
ARR, ChIP, WB
H7162 Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
mouse, Caenorhabditis elegans, rat, bovine, chicken, frog, human, Drosophila
ARR, WB
H0412 Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
human, mouse, frog, Drosophila, rat, Caenorhabditis elegans, bovine, chicken
ARR, IF, WB
H9908 Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat HTA28, monoclonal
purified immunoglobulin
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
human, bovine, hamster, mouse
ARR, FACS, ICC, WB
H9289 Anti-Histone H3 (N-terminal) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST1H3A(8350)
human
IF, WB
M4819 Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
chicken, Caenorhabditis elegans, mouse, bovine, Drosophila, human, frog, Arabidopsis thaliana, rat
WB
H7664 Anti-phospho-Histone H1.4 (pSer27) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HIST1H1E(3008)
human
WB