Antibodies to Modifying Enzymes/Histone Deacetylase

Regulation of gene expression is mediated by several mechanisms including DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones. One of the major modifications of histones consists of the dynamic acetylation and deacetylation of ε-amino groups of lysine residues present in the tail of core histones.1 The enzymes responsible for this reversible acetylation/deacetylation process are histone acetyltransferases (HATs) and histone deacetylases (HDACs), respectively.3

Mammalian HDACs can be divided into three classes according to sequence homology.4 Class I consists of the yeast Rpd3-like proteins HDAC1, HDAC2, HDAC3 and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10.5 Class III comprises the yeast Sir2-like proteins. Whereas class I HDACs are ubiquitously expressed, most class II HDACs are tissue-specific. Sir2 is an NAD-dependent histone deacetylase that appears to play an important role in chromatin silencing and life span prolongation through calorie restriction.6

Sigma® has a wide selection of antibodies to many of the histone modifying enzymes. The antibodies are carefully tested to provide high quality performance. These antibodies are valuable tools for scientists working on chromatin remodeling.


References

1. Wang, A.H., et al. Mol. Cell. Biol., 19, 7816-7827 (1999).
2. Grozinger, C.M. et al. Proc. Natl. Acad. Sci. USA, 96, 4868-4873 (1999).
3. Fischle, W., et al. Biochem. Cell Biol., 79, 337 (2001).
4. Khochbin, S., et al. Curr. Opin. Genet. Dev., 11, 162-166 (2001).
5. Fischle, W., et al. J. Biol. Chem., 274, 11713-11720 (1999).

Product #

Description

Clone

Form

Gene Symbol (ID)

Species Reactivity

Application

Add to Cart

HPA029693 Anti-HDAC1 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC1(3065)
human
IF, IHC
WH0055869M1 Monoclonal Anti-HDAC8 antibody produced in mouse 2F4, monoclonal
purified immunoglobulin
human ... HDAC8(55869)
mouse, human, rat
ELISA (i), IF, WB
HPA028926 Anti-HDAC9 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC9(9734)
human
IF, IHC
H3284 Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... HDAC1(3065)
mouse ... Hdac1(433759)
mouse, human
ARR, IHC (p), IP, WB
H3159 Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... HDAC2(3066)
mouse ... Hdac2(15182)
mouse, human
ARR, IHC (p), IP, WB
H3034 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... HDAC3(8841)
mouse ... Hdac3(15183)
rat ... Hdac3(84578)
rat, human, mouse
ARR, IHC (p), IP, WB
H6537 Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse HDAC3-83, monoclonal
purified immunoglobulin
human ... HDAC3(8841)
mouse ... Hdac3(15183)
human, mouse
ARR, WB
H9536 Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
rat, human, mouse
ARR, IF, IP, WB
H9411 Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
rat, mouse, human
ARR, IF, IP, WB
H0163 Monoclonal Anti-Histone Deacetylase 4 (HDAC4) antibody produced in mouse HDAC4-144, monoclonal
purified immunoglobulin
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
mouse, human, rat
ICC, IP, WB
H8163 Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)
rat, mouse, human
ARR, ICC, IHC, IP, WB CL
H2287 Anti-Histone Deacetylase 6 (HDAC6) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... HDAC6(10013)
mouse ... Hdac6(15185)
human, mouse
ARR, IP, WB
H2662 Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC7(51564)
mouse ... Hdac7(56233)
rat ... Hdac7a(84582)
rat, human, mouse
IF, IP, WB
H6663 Monoclonal Anti-Histone Deacetylase 7 (HDAC7) antibody produced in mouse HDAC7-97, monoclonal
purified immunoglobulin
human ... HDAC7(51564)
mouse ... Hdac7(56233)
mouse, human
ARR, ELISA (i), ICC, IP, WB
H2537 Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC7(51564)
mouse ... Hdac7(56233)
rat ... Hdac7a(84582)
rat, human, mouse
ARR, IP, WB
H8038 Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC8(55869)
mouse ... Hdac8(70315)
human, mouse
ICC, IP, WB
H6412 Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse HDAC8-48, monoclonal
purified immunoglobulin
human ... HDAC8(55869)
human
ARR, ELISA (i), WB
H3413 Anti-Histone Deacetylase 10 (HDAC10) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC10(83933)
mouse ... Hdac10(170787)
rat ... Hdac10(362981)
mouse, human, rat
ARR, IF, WB CL
H4539 Anti-Histone Deacetylase 11 (HDAC11) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HDAC11(79885)
mouse ... Hdac11(232232)
rat ... Hdac11(297453)
mouse, human
WB CL
H6287 Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal HDAC1-21, monoclonal
purified immunoglobulin
human ... HDAC1(3065)
mouse ... Hdac1(433759)
mouse, human
ARR, ELISA (i), IP, WB
H2663 Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal HDAC2-62, monoclonal
purified immunoglobulin
human ... HDAC2(3066)
mouse ... Hdac2(15182)
rat ... Hdac2(84577)
canine, mouse, rat, chicken, human, bovine
ARR, ELISA (i), IHC, IP, WB
H4538 Monoclonal Anti-Histone Deacetylase 5 (HDAC5) antibody produced in mouse HDAC5-35, monoclonal
purified immunoglobulin
human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)
mouse, rat, human
ARR, ELISA (i), ICC, IP, WB
S5313 Anti-Sir2 (AS-16) antibody produced in rabbit polyclonal
affinity isolated antibody
mouse ... Sirt1(93759)
mouse
ARR, IF, WB
S5447 Anti-SIRT1 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)
human
IP, WB
S5196 Monoclonal Anti-Sirt1 antibody produced in mouse SIR11, monoclonal
purified immunoglobulin
mouse ... Sirt1(93759)
mouse
ARR, ELISA (i), WB
S5322 Anti-Sirt1 (C-terminal) antibody produced in rabbit polyclonal
affinity isolated antibody
human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)
human
IF, IP, WB
SAB2500945 Anti-SIRT3 antibody produced in goat polyclonal
affinity isolated antibody
human ... SIRT3(23410)
human
ELISA (i), WB
S4072 Anti-Sirt3 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... SIRT3(23410)
human
IF, WB
HPA026809 Anti-SIRT3 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... SIRT3(23410)
human
IHC
AV32388 Anti-SIRT3 (AB2) antibody produced in rabbit polyclonal
IgG fraction of antiserum
human ... SIRT3(23410)
rabbit, rabbit, zebrafish, guinea pig, rat, human, mouse, canine, horse
WB
S5947 Anti-Sirt7 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... SIRT7(51547)
mouse ... Sirt7(209011)
human, mouse (predicted)
IF, IP, WB
AV32406 Anti-SIRT7 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... SIRT7(51547)
pig, rat, mouse, horse, guinea pig, human, canine, bovine
WB