Antibodies to Peptide and Protein Fusion Tags

Recombinant DNA technology enables the insertion of specific foreign sequences to genes of interest. When these sequences encode short peptides they create an antigenic determinant (epitope) that can be recognized by antibodies. Thus, when the DNA sequence of interest is fused with the DNA sequence of the short peptide and introduced into cells, the resulting expressed protein is now a "tagged protein." Since antibodies to the peptide tag are commercially available, there is no need to generate specific antibodies to identify, immunoprecipitate or immunoaffinity purify the protein. Moreover, in most cases the stable fusion protein has the same bioactivity and biodistribution as the native protein.

Many different epitope tags have been engineered into recombinant proteins. These include FLAG®, HA, HIS, c-Myc, VSV-G, V5 and HSV.

Due to the importance of this technology for protein expression, Sigma® offers a wide range of products for the detection, isolation and purification of tagged proteins. These include monoclonal and polyclonals antibodies specific for the most commonly used epitope tags, as well as antibody-enzyme conjugates, fluorescent antibodies, antibody-affinity resins and the specific peptide tag sequence. Our products are carefully tested in many applications to ensure the most highly efficient performance.

Product #

Product Name

Antibody Form

Application

Add to Cart

P2983 ANTI-FLAG® High Sensitivity, M2 coated 96-well plates 96-well, clear, polystyrene, flat bottom plate   ELISA
A4596 ANTI-FLAG® M1 Agarose Affinity Gel    
A2220 ANTI-FLAG® M2 Affinity Gel purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
 
F7425 ANTI-FLAG® antibody produced in rabbit affinity isolated antibody, buffered aqueous solution affinity isolated antibody
DB, IF, IP, WB CL
SAB4200071 ANTI-FLAG® antibody, Rat monoclonal clone 6F7, purified from hybridoma cell culture purified immunoglobulin
IP, WB
G7781 Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution IgG fraction of antiserum
DB, ELISA (i), WB
A8580 Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit IgG fraction of antiserum, PBS suspension IgG fraction of antiserum
ELISA, IP
A5838 Anti-Glutathione-S-Transferase (GST)–Alkaline Phosphatase Conjugate antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution IgG fraction of antiserum
ELISA (d), WB CL
A7340 Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution IgG fraction of antiserum
ELISA (d), WB
A2582 Anti-Thioredoxin–Agarose antibody produced in rabbit IgG fraction of antiserum, PBS suspension IgG fraction of antiserum
 
A1970 Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal clone P5D4, purified from hybridoma cell culture, PBS suspension purified immunoglobulin
 
AV41959 Anti-ALPP antibody produced in rabbit IgG fraction of antiserum, lyophilized powder IgG fraction of antiserum
IHC, WB
11814460001 Anti-GFP from mouse IgG1κ (clones 7.1 and 13.1) purified immunoglobulin
 
16-252 Anti-GST Tag Antibody, Alexa Fluor® 488 conjugate 50 µg, Anti-GST Tag Antibody, Alexa Fluor 488 conjugate is an antibody against GST Tag for use in IF, WB & IC.    
06-332 Anti-GST Tag Antibody 200 µg, Anti-GST Tag Antibody detects level of GST Tag & has been published & validated for use in WB.    
MAC111 Anti-GST•Tag™ fusion protein Antibody 50 µg, This Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.    
11815016001 Anti-HA Affinity Matrix from rat IgG1 purified immunoglobulin
 
H6908 Anti-HA antibody produced in rabbit affinity isolated antibody, buffered aqueous solution affinity isolated antibody
IF, IP, WB
MAC123 Anti-HSV•Tag® Antibody, clone HSV 78 100 µg, This HSV•Tag antibody is validated for use in WB for the detection of the HSV•Tag protein.    
11815024001 Anti-Protein C Affinity Matrix from mouse IgG1κ purified immunoglobulin
 
MAC112 Anti-S•Tag™ fusion protein Antibody 50 µg, Detect S•Tag fusion protein using this Anti-S•Tag fusion protein Antibody validated for use in Western Blotting, ICC, Immunoprecipitation.    
AB3790 Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) 100 µg, Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.    
MAC113 Anti-T7•Tag® fusion protein Antibody 50 µg, Anti-T7•Tag fusion protein Antibody detects level of T7•Tag fusion protein & has been published & validated for use in Western Blotting.    
T0803 Anti-Thioredoxin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution IgG fraction of antiserum
DB, WB
A7345 Anti-V5 Agarose Affinity Gel antibody produced in mouse purified immunoglobulin, clone V5-10 purified immunoglobulin
 
V8137 Anti-V5 antibody produced in rabbit IgG fraction of antiserum IgG fraction of antiserum
ICC, IP, WB
C3956 Anti-c-Myc antibody produced in rabbit ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution affinity isolated antibody
ARR, ICC, IF, IP, WB
A5963 Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal clone 9E10, purified from hybridoma cell culture purified immunoglobulin
ELISA, IHC (p), WB
A5598 Anti-c-Myc−Peroxidase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution affinity isolated antibody
WB
FLAGIPT1 FLAG® Immunoprecipitation Kit    
71097 GST•Tag™ Monoclonal Antibody Sensitive, specific detection of GST•Tag fusion proteins    
F9291 Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
DB
F3040 Monoclonal ANTI-FLAG® M1 antibody produced in mouse clone M1, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
WB CL
F3165 Monoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
WB CL
A9469 Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
ELISA (i)
A9594 Monoclonal ANTI-FLAG® M2-Cy3 antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate,) purified immunoglobulin
IF (d)
F4049 Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
IF (d)
A8592 Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
ELISA (d)
F4042 Monoclonal ANTI-FLAG® M5 antibody produced in mouse clone M5, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
WB CL
F2555 Monoclonal ANTI-FLAG® antibody produced in rabbit clone SIG1-25, ascites fluid ascites fluid
ELISA (i), ICC, WB
SAB4600371 Monoclonal Anti-6X His IgG, CF680 antibody produced in mouse ~2 mg/mL, clone 33D10.D2.G8, affinity isolated antibody, buffered aqueous solution affinity isolated antibody
IF, WB
SAB4600386 Monoclonal Anti-6X His IgG, CF770 antibody produced in mouse ~2 mg/mL, clone 33D10.D2.G8, affinity isolated antibody, buffered aqueous solution affinity isolated antibody
IF, WB
G1160 Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse clone GST-2, ascites fluid ascites fluid
DB, ELISA (i), WB
SAB4200055 Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in rat clone GST-R 6G9, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
ELISA (i), IP, WB
A5713 Monoclonal Anti-polyHistidine–Agarose antibody produced in mouse clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins purified immunoglobulin
 
A2951 Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse clone 8B6, ascites fluid ascites fluid
ELISA (i), ICC, IHC (f), IHC (p), RIA, WB
A2080 Monoclonal Anti-Alkaline Phosphatase, Human Placental−Agarose antibody produced in mouse clone 8B6, purified immunoglobulin, PBS suspension purified immunoglobulin
IP
B6804 Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse clone BAP-77, ascites fluid ascites fluid
ELISA (i), WB
SAB4200119 Monoclonal Anti-FLAG-Peroxidase antibody produced in rat 2-4 mg/mL, clone 6F7, purified immunoglobulin purified immunoglobulin
WB
G6539 Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GFP-20, ascites fluid ascites fluid
DB, ELISA (i), WB
G1546 Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GSN149, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
ELISA (i), WB
H9658 Monoclonal Anti-HA antibody produced in mouse clone HA-7, ascites fluid ascites fluid
ELISA (i), ICC, IP, WB
A2095 Monoclonal Anti-HA−Agarose antibody produced in mouse clone HA-7, purified immunoglobulin, PBS suspension purified immunoglobulin
 
SAB4200082 Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat 1.0 mg/mL, clone MBP 7G4, purified immunoglobulin purified immunoglobulin
ELISA (i), IP, WB
M6295 Monoclonal Anti-Maltose Binding Protein antibody produced in mouse clone MBP-17, ascites fluid ascites fluid
DB, ELISA (i), WB
M1321 Monoclonal Anti-Maltose Binding Protein antibody produced in mouse clone MBP-17, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
DB, ELISA (i), WB
A3963 Monoclonal Anti-Maltose Binding Protein−Alkaline Phosphatase antibody produced in mouse clone MBP-17, purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
WB
A4213 Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse clone MBP-17, purified immunoglobulin, lyophilized powder purified immunoglobulin
WB
V8012 Monoclonal Anti-V5 antibody produced in mouse ~1 mg/mL, purified immunoglobulin, clone V5-10 purified immunoglobulin
ICC, WB
V2260 Monoclonal Anti-V5-Peroxidase antibody produced in mouse clone V5-10, purified immunoglobulin, lyophilized powder purified immunoglobulin
WB
V5507 Monoclonal Anti-VSV Glycoprotein antibody produced in mouse clone P5D4, ascites fluid ascites fluid
ICC, IP, WB
C7706 Monoclonal Anti-VSV Glycoprotein−Cy3 antibody produced in mouse clone P5D4, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
IF (d)
A5977 Monoclonal Anti-VSV-G−Peroxidase antibody produced in mouse 1.0-1.5 mg/mL, clone P5D4, purified immunoglobulin, lyophilized powder purified immunoglobulin
WB
SAB3300002 Monoclonal Anti-c-Myc antibody produced in mouse clone 1A6, purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
WB
M5546 Monoclonal Anti-c-Myc antibody produced in mouse clone 9E10, ascites fluid ascites fluid
ELISA (i), ICC, IHC (p), IP, WB
M4439 Monoclonal Anti-c-Myc antibody produced in mouse clone 9E10, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
ARR, ICC, IHC (p), IP, WB
B7554 Monoclonal Anti-c-Myc−Biotin antibody produced in mouse clone 9E10, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
WB CL
C6594 Monoclonal Anti-c-Myc−Cy3 antibody produced in mouse clone 9E10, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
IF (d)
F2047 Monoclonal Anti-c-Myc−FITC antibody produced in mouse clone 9E10, purified immunoglobulin, buffered aqueous solution purified immunoglobulin
ICC
H1029 Monoclonal Anti-polyHistidine antibody produced in mouse clone HIS-1, ascites fluid ascites fluid
DB, ELISA (i), IP, WB
A5588 Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse clone HIS-1, purified immunoglobulin, buffered aqueous glycerol solution purified immunoglobulin
WB
A7058 Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse clone HIS-1, purified immunoglobulin, lyophilized powder purified immunoglobulin
WB
481409 PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda