Human IgG Subclasses

Sigma offers human IgG subclasses as purified immunoglobulins. Each purified immunoglobulin represents a single subclass and light chain type. Human IgG subclasses have been isolated from myeloma plasma by fractionation, ion-exchange chromatography, and/or affinity chromatography. The identity and purity of the immunoglobulins are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE. Protein content is determined by spectrophotometry. The antigen specificity of these myelomas has not been determined. The source material for human IgG subclasses has been tested and found negative for antibody to HIV and HCV, and for HbsAG.

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I5154 IgG1, Kappa from human myeloma plasma purified immunoglobulin, >95% (SDS-PAGE)
I5029 IgG1, Lambda from human myeloma plasma purified immunoglobulin, >95% (SDS-PAGE)
I5404 IgG2, Kappa from human myeloma plasma purified immunoglobulin, >95% (SDS-PAGE)
I5279 IgG2, Lambda from human myeloma plasma purified immunoglobulin, >95% (SDS-PAGE)
I5654 IgG3, Kappa from human myeloma plasma purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis)
I5529 IgG3, Lambda from human myeloma plasma purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis)
I4639 IgG4, Kappa from human myeloma plasma purified immunoglobulin, >95% (SDS-PAGE)
I4764 IgG4, Lambda from human myeloma plasma purified immunoglobulin, >95% (SDS-PAGE)
K3388 Kappa Light Chains (Free) from human myeloma plasma purified immunoglobulin, ~1.0 mg/mL, ≥99% (electrophoresis), buffered aqueous solution
L0665 Lambda Light Chains (Free) from human myeloma plasma purified immunoglobulin, ~1.0 mg/mL, ≥99% (electrophoresis), buffered aqueous solution