Purified Immunoglobulins

Purified myeloma immunoglobulins are isolated from ascites fluid by a combination of fractionation, ion-exchange chromatography, and/or affinity chromatography. The identity of the immunoglobulins is confirmed by immunoelectrophoresis against anti-whole serum and anti-subclass specific antisera. Ouchterlony Double Diffusion against subclass specific antisera is used to confirm the absence of contaminating subclasses. Purity of the immunoglobulin is determined by SDS-PAGE. Protein content is determined by spectrophotometry. For applications that may be affected by the presence of azide, the azide may be removed by dialyzing the protein against the desired buffer. Sigma offers a disposable dialyzer (Z368393) with a capacity of 2 mL and a molecular weight cutoff of 10,000 that is suitable for dialysis of the purified myeloma immunoglobulins.

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M1421 IgA, Kappa from murine myeloma clone TEPC 15, purified immunoglobulin, buffered aqueous solution
M9269 IgG1, Kappa from murine myeloma clone MOPC 21, purified immunoglobulin, buffered aqueous solution
M9035 IgG1, Kappa from murine myeloma clone MOPC 31C, purified immunoglobulin, buffered aqueous solution
M9144 IgG2a, Kappa from murine myeloma clone UPC 10, purified immunoglobulin, buffered aqueous solution
M6034 IgG2a, Lambda from murine myeloma clone HOPC-1, purified immunoglobulin, buffered aqueous solution
M8894 IgG2b, Kappa from murine myeloma clone MOPC 141, purified immunoglobulin, buffered aqueous solution
M9019 IgG3, Lambda from murine myeloma clone Y5606, purified immunoglobulin, buffered aqueous solution
M3795 IgM, Kappa from murine myeloma clone TEPC 183, purified immunoglobulin, buffered aqueous solution
M5170 IgM, Lambda from murine myeloma clone MOPC 104E, purified immunoglobulin, buffered aqueous solution