Protein A, G and L Resins

Protein A and Protein G have binding sites for the Fc portion of mammalian IgG. The capacity of these proteins for IgG varies with the species. In general, IgGs have a higher affinity for Protein G than for Protein A, and Protein G can bind IgG from a wider variety of species. The affinity of various IgG subclasses, especially from mouse and human, for Protein A varies more than for Protein G. Protein A can, therefore, be used to prepare isotypically pure IgG from some species. Protein L has an affinity for kappa light chains from various species. It can be used to purify monoclonal or polyclonal IgG, IgA, and IgM as well as Fab, F(ab′)2, and recombinant scFv fragments that contain kappa light chains. Note: Species such as bovine, goat, sheep, and horse whose Igs contain almost exclusively lambda chains will not bind well, if at all, to Protein L. This makes it suitable for isolating mouse monoclonal antibodies from cell culture supernatants without contamination by bovine IgG.
See also Protein A, G, and L Detection Reagents.

Product #

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GE17-5478-01 Capto L GE Healthcare, 17-5478-01, pack of 25 mL
GE17-5478-02 Capto L GE Healthcare, 17-5478-02, pack of 200 mL
P6486 EZview Red Protein A Affinity Gel
E3403 EZview Red Protein G Affinity Gel
GE17-0886-01 Gammabind Plus Sepharose® GE Healthcare, 17-0886-01, pack of 5 mL
GE17-0886-02 Gammabind Plus Sepharose® GE Healthcare, 17-0886-02, pack of 25 mL
GE17-0886-04 Gammabind Plus Sepharose® GE Healthcare, 17-0886-04, pack of 500 mL
GE17-0885-01 Gammabind G Sepharose® GE Healthcare, 17-0885-01, pack of 5 mL
GE17-0885-02 Gammabind G Sepharose® GE Healthcare, 17-0885-02, pack of 25 mL
GE29-0491-04 HiTrap® MabSelect SuRe GE Healthcare, 29-0491-04, pack of 1 mL
GE29-0486-65 HiTrap Protein L GE Healthcare, 29-0486-65, pack of 1 mL
54838 HiTrap® Protein A Cartridge part volume 1 mL
54840-U HiTrap® Protein G Cartridge part volume 1 mL
GE29-0485-81 HiTrap® Protein G HP GE Healthcare, 29-0485-81, pack of 1 mL
GE17-6002-35 Immunoprecipitation Starter Pack GE Healthcare, 17-6002-35, pack of 1 kit
GE17-5199-02 MabSelect GE Healthcare, 17-5199-02, pack of 200 mL
GE17-5199-01 MabSelect GE Healthcare, 17-5199-01, pack of 25 mL
GE17-5438-01 MabSelect SuRe GE Healthcare, 17-5438-01, pack of 25 mL
GE17-5438-02 MabSelect SuRe GE Healthcare, 17-5438-02, pack of 200 mL
GE17-5474-01 MabSelect SuRe LX GE Healthcare, 17-5474-01, pack of 25 mL
GE17-5474-02 MabSelect SuRe LX GE Healthcare, 17-5474-02, pack of 200 mL
GE17-5269-07 MabSelect Xtra GE Healthcare, 17-5269-07, pack of 25 mL
GE17-5269-02 MabSelect Xtra GE Healthcare, 17-5269-02, pack of 200 mL
GE17-5280-01 nProtein A Sepharose® 4 Fast Flow GE Healthcare, 17-5280-01, pack of 5 mL
GE17-5280-02 nProtein A Sepharose® 4 Fast Flow GE Healthcare, 17-5280-02, pack of 200 mL
GE17-5280-04 nProtein A Sepharose® 4 Fast Flow GE Healthcare, 17-5280-04, pack of 25 mL
P2545 Protein A–Agarose saline suspension
P1406 Protein A–Agarose lyophilized powder
P9269 Protein A–Agarose lyophilized powder
P7786 Protein A-Agarose from Staphylococcus aureus aqueous suspension
P0932 Protein A-Agarose from Staphylococcus aureus lyophilized powder
P3476 Protein A–Agarose Fast Flow 50%, aqueous suspension
P2670 Protein A (extracellular)–Agarose from Staphylococcus aureus saline suspension
P5906 Protein A (extracellular)–Agarose from Staphylococcus aureus lyophilized powder
P1925 Protein A–Agarose macrobeads aqueous suspension
P3391 Protein A-Sepharose® from Staphylococcus aureus lyophilized powder
P6649 Protein A–Sepharose® 6MB aqueous ethanol suspension
P9424 Protein A-Sepharose® 4B, Fast Flow from Staphylococcus aureus aqueous ethanol suspension
83219 Protein G, immobilized on cross-linked 4% agarose
P3296 Protein G Sepharose®, Fast Flow recombinant, expressed in E. coli, aqueous ethanol suspension
P3351 Protein L–Agarose from Peptostreptococcus magnus recombinant, expressed in E. coli
GE28-9852-55 Protein G GraviTrap GE Healthcare, 28-9852-55, pack of 10 × 1 mL
GE17-0469-01 Protein A-Sepharose® 6MB GE Healthcare, 17-0469-01
GE17-0963-02 Protein A Sepharose® Cl-4B GE Healthcare, 17-0963-02, pack of 500 mL
GE17-0963-03 Protein A Sepharose® Cl-4B GE Healthcare, 17-0963-03, pack of 25 mL
GE17-0780-01 Protein A-Sepharose® CL-4B GE Healthcare, 17-0780-01, pack of 1.5 g
GE17-0618-01 Protein G Sepharose® 4 Fast Flow GE Healthcare, 17-0618-01, pack of 5 mL
GE17-0618-02 Protein G Sepharose® 4 Fast Flow GE Healthcare, 17-0618-02, pack of 25 mL
GE17-0618-05 Protein G Sepharose® 4 Fast Flow GE Healthcare, 17-0618-05, pack of 200 mL
GE17-5138-03 rmp Protein A Sepharose® Fast Flow GE Healthcare, 17-5138-03, pack of 200 mL
GE17-5138-01 rmp Protein A Sepharose® Fast Flow GE Healthcare, 17-5138-01, pack of 5 mL
GE17-1279-01 rProtein A Sepharose® Fast Flow GE Healthcare, 17-1279-01, pack of 5 mL
GE17-1279-02 rProtein A Sepharose® Fast Flow GE Healthcare, 17-1279-02, pack of 25 mL
GE17-1279-03 rProtein A Sepharose® Fast Flow GE Healthcare, 17-1279-03, pack of 200 mL