Sigma Life Science offers a portfolio of polyclonal and monoclonal Actin antibodies. Select appropriate Actin antibodies for your research by form, gene symbol, species reactivity or application. Each Actin antibody is covered by our Bioguarantee so that you can evaluate our Actin antibodies with complete peace of mind.
The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Actin is one of the most conserved eukaryotic proteins with at least six isoforms. Four of the isoforms represent the differentiation markers of muscle tissues and two are found in almost all cells. There are three a-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Actin isoforms show greater than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. The NH2-terminal region of actin appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Note: Historically, due to its ubiquitous nature, β-Actin was used as an internal standard for Western blotting. More recently its levels have been shown to fluctuate following different treatments. Therefore, GAPDH is becoming more popular as a housekeeping gene.