Duolink® & PLA® Technology

Duolink based on Proximity Ligation Assay (in situ PLA) technology when combined with qualified antibodies outperforms traditional immunoassays, such as co-IP, through a simple and sensitive in situ detection of endogenous proteins. This technology enables you to visualize protein interactions in fixed cells and tissue samples, all while under endogenous protein expression.

  • Visualize individual interactions without having to overexpress proteins
  • Gain high specificity with dual binding of primary antibodies
  • Single molecule sensitivity due to signal amplification
  • Analyze using standard immunofluorescence instruments
  • Amenable to high throughput cell-based screens





PLA Validated Antibodies

Proximity Ligation Assay (PLA) pre-qualified primary antibodies and primary antibody pairs (kits) provide the researcher a set of high quality validated primary antibodies for use in PLA.

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If you have questions regarding Duolink and Proximity Ligation Assay Technology, feel free to reach out to by clicking the forms below.




 Literature & Resources

 
View the Workflow
View the steps for preparing, detecting and analyzing proteins using Duolink and PLA technology. Download our brochure for an overview of the technology.
 
 
View On-Demand Webinars
Click here to view our on-demand webinars to learn about introducing PLA into your research projects.

 
 
Search Our FAQs
Check to see if your questions have been answered in our FAQ document. Or, browse our Tips & Tricks (PDF) for additional insights.