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Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human diseases, such as Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury. In light of its highly reactive nature, evidence for a role of peroxynitrite in vivo is largely based on the detection of 3-nitrotyrosine, the major product of the peroxynitrite reaction, in injured tissues.
Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates.
Applications: immunoblotting, ELISA, immunohistochemistry and immunofluorescence.
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