Protein-G Immunoprecipitation Kit

Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. IP followed by SDS-PAGE and immunoblotting is routinely used to: measure the molecular weights of protein antigens, study protein/protein interactions, determine specific enzymatic activity, monitor protein post-translational modifications and determine the presence and quantity of proteins. IP also enables the detection of rare proteins that otherwise would be difficult to detect, since immunoprecipitation can concentrate them as much as 10,000 fold.

The kit has several advantages:

• Allows for maximum recovery of immunoprecipitates
  
• Uses mini-spin columns, instead of microcentrifuge tubes
  
• Easy and convenient washing of the antigen-antibodybound beads
  
• Minimal loss of antigen/antibody bound beads during washing
  
• Minimal or no non-specific signals by increasing the stringency of the washing steps
  

Sufficient for performing 50 IP assays.

Figure 1. Procedure Diagram

Components

• 10X IP buffer, 40 ml
• 5 M NaCl, 15 ml
• 10% SDS, 1 ml
• Protein-G-Agarose, suspension, 2.0 ml
• Spin columns and caps, 50 units
• 2 ml Microcentrifuge tubes, 50 units

Product Table

back to Spotlights
back to Antibody Explorer
back to top