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Antibody Explorer
What's New with Sigma® Antibodies?
Be the first to know about antibody updates and exclusive offers! Sign up for alerts. SEPTEMBER 2011 UPDATES Last updated September 12, 2011 Webinar: On September 28, 2011 Sigma® Life Science will be hosting a webinar through Biocompare highlighting the use of Prestige Antibodies® produced by the Human Protein Atlas project in subcellular protein localization studies. Title: Creation of an Antibody-based Subcellular Protein Atlas About Dr. Lundberg About the abstract: Creation of an Antibody-based Subcellular Protein Atlas Protein localization on a subcellular level is essential to map and characterize the human proteome and to better understand the cellular functions of proteins. Confocal microscopy is a powerful method for studying the subcellular distribution of proteins, yielding high-resolution images of the most intricate cellular substructures. Antibodies produced in the Human Protein Atlas project are used to systematically localize proteins in three human cell lines of functionally different origin1,2. Four-color images of the protein of interest and markers for nuclei, microtubules, and endoplasmic reticulum are acquired using high-resolution confocal microscopy. All images are manually annotated in terms of protein distribution, staining intensity, and characteristics. In total, 17 different subcellular locations are annotated. We will discuss the development and progression of a subcellular protein atlas, as part of the Human Protein Atlas portal (proteinatlas.org). Currently, the subcellular data comprises >50,000 high-resolution, annotated confocal images corresponding to the analysis of over 8,000 proteins in the three human cell lines. A global analysis reveals that 85% of the examined proteins are clearly localized, indicating that antibody-based subcellular profiling is a viable approach3. As much as 35% of proteins localize to multiple subcellular compartments, reflecting the dynamic behavior of cells. Hierarchical clustering reveals distinct organelle proteomes, common for all three cell lines and a strong correlation to protein function. Further, we present the development of a platform for systematic validation of antibody specificity and protein subcellular location using siRNA. The presented atlas aims to provide publicly available information on subcellular information for all human proteins with the ultimate aim to facilitate functional studies of proteins. References
AUGUST 2011 UPDATES Last updated August 19, 2011 Over 600 New Antibodies Validated for flow Cytometry At Sigma Life Science, we have added over 600 monoclonal antibodies specifically validated for flow cytometry. These antibodies are available in purified form or conjugated to some of the most popular fluorochromes.
You can choose from a broad selection of markers for:
All antibodies are Bioguaranteed and can be easily accessed via the Antibody Explorer Search Tool. Find your antibody today at sigma.com/antibodyexplorer.
Flow cytometry analysis of human peripheral blood cells with anti-human CD4 (MEM-241) FITC. (Product No. SAB4700062) Featured Article: New Cancer Biomarker Discovered by the use of Prestige Antibodies®, Powered by Atlas Antibodies The RNA binding protein, RBM3, was initially identified as a potential biomarker within the Human Protein Atlas (HPA) project. New data from extended Tissue Microarrays (TMAs) show that RBM3 is a biomarker of good prognosis in several cancer forms.
Immunohistochemical analysis using the Anti-RBM3 antibody (HPA003624) shows weak expression in normal breast tissue and differential expression, varying from weak to strong, in tumor breast samples. Learn more: New Cancer Biomarker Discovered (1.41 Mb PDF) JULY 2011 UPDATES Last updated July 8, 2011 140 New Highly Validated Antibodies Our antibody collection, at Sigma Life Science, continues to expand. Over 140 rabbit polyclonal antibodies have just been released.
All antibodies are Bioguaranteed* and can be found using our Antibody Explorer Search Tool. Search by antigen, application, gene, disease state and more. View our new products at wherebiobegins.com/antibodyexplorer. *Experimental results must be submitted via the Antibody Bioguarantee Form within 12 months of the date of purchase. All required fields of the Antibody Bioguarantee Form must be completed. Refunds and replacements contingent to claim review by technical service team. Credit covers the cost of antibody. Product replacements depend on product availability. Antibodies purchased in bulk order or for resale purposes are expressly excluded from this Bioguarantee. This Bioguarantee is non-transferable and void upon resale of antibody. Featured Article: Explore Proteins of Unknown Function by the Use of Prestige Antibodies®, Powered by Atlas Antibodies Over 26% of the Prestige Antibody catalog contains antibodies directed towards uncharacterized proteins. The development of these antibodies has been instrumental at unlocking information regarding basic function and potential roles of these proteins.
In the figure above, two different Prestige Antibodies generated towards a putative protein encoded by the C9orf11 gene, for which there is only evidence of existence on a transcript level, show a highly specific immunoreactivity in the innermost layer of maturing cells in the seminiferous ducts of testis. The localization of immunoreactivity is well in accordance with the acrosome and thus these results indicate that this protein is involved in sperm maturation and function. Learn more: MAY 2011 UPDATES Last updated May 13, 2011 7,000 New Antibodies Sigma Life Science has just added 7,000 monoclonal and polyclonal antibodies to our world-class antibody collection.
All antibodies are Bioguaranteed* and can be found using our Antibody Explorer Search Tool. Search by antigen, application, gene, disease state and more. View our new products at wherebiobegins.com/antibodyexplorer. 1,400 Exclusive Prestige Antibodies® Powered by Atlas Antibodies Just Released Prestige Antibodies are the most highly characterized in the industry. Through our exclusive partnership with Atlas Antibodies, Sigma Life Science has now added 1,400 new antibodies to our portfolio Each antibody is:
All data is publically available on the Human Protein Atlas website. Learn more about Prestige antibodies at wherebiobegins.com/prestige.
Featured Article: Prestige Antibodies, Powered by Atlas Antibodies in Immunofluorescence Applications Immunofluorescence has proven to be a key technique in learning more about protein function and interaction. To date, 1800 Prestige antibodies are approved for the IF application. Learn more about how Prestige antibodies are used in subcellular localization studies.
Figures A-H: Diversity of nuclear staining patterns shown by eight different antibodies in cell line U2-OS or A-431. A) Smooth nuclear staining by anti-MECP2 (HPA000593), B) nuclear staining without nucleoli by anti-RBM14 (HPA006628), C) nucleoli staining by anti-ZSCAN1 (HPA007938), D) cluster staining within nucleoli by anti-MUC4 (HPA005895), E) nuclear membrane staining by anti-UNC84B (HPA001209), F) nuclear granular staining by anti-BCLAF1(HPA006669) G) nuclear speckled staining by anti-RBM25 (HPA003025), H) nuclear body (SMN) staining by anti-DDX20 (HPA005516). Access the article today at: Prestige Antibodies in IF Applications MARCH 2011 UPDATES March 5, 2011 Video: Antibodies are Where Bio Begins! Tracey Harshberger
Global Product Manger for Antibodies Shares how Sigma Life Science has made finding antibodies easy. Evaluate Antibody Products with Complete Peace of Mind
We invite you to evaluate our antibody products with complete peace of mind. If our antibody does not perform in your application of interest, or species specificity, we will issue a full credit or replacement antibody product – Bioguaranteed* Learn more about our industry leading, no hassle Bioguarantee program at: *Experimental results must be submitted via the Antibody Bioguarantee Form within 12 months of the date of purchase. All required fields of the Antibody Bioguarantee Form must be completed. Refunds and replacements contingent to claim review by technical service team. Credit covers the cost of antibody. Product replacements depend on product availability. Antibodies purchased in bulk order or for resale purposes are expressly excluded from this Bioguarantee. This Bioguarantee is non-transferable and void upon resale of antibody.
Our NEW Kinase Phosphatase Biology Product Guide features a collection of biomolecules for all your kinase biology research needs, including 300 NEW Phospho-specific antibodies. For a limited time. Purchase any product featured in the guide and receive your FREE bioperformance t-shirt. To request your copy and learn more visit wherebiobegins.com/kinasebiology. FEBRUARY 2011 UPDATES February 4, 2011 Now Offering over 40,000 Antibodies! – Sigma Life Science is rising to the top with a continuously growing world-class antibody collection Comprehensive collection in a variety of host species
Meticulous application testing and validation
Unsurpassed quality standards, search-ability, and support
2010 UPDATES November 15, 2010 Addition of 1,700 New Prestige Antibodies® powered by Atlas Antibodies
Discover how Prestige Antibodies could prove to be priceless to your research at sigma.com/prestige . Learn more about the Human Protein Atlas Project and Prestige Antibodies at the upcoming "Towards a Knowledge-based Human Protein Atlas" webinar on December 8, 2010 at 11:00 AM CST. For more information or to register visit wherebiobegins.com/symposium . August 8, 2010 New Antibody Explorer Search Tool Our New Antibody Explorer provides you easy access to our growing collection of world-class antibodies that you need for your research program. The convenient, expanded search capabilities allows you to:
Search for your antibody today at sigma.com/antibodyexplorer. Three New White Papers featuring Prestige Antibodies® powered by Atlas Antibodies Prestige Antibodies are the most highly characterized in the industry!
White Papers are now available highlighting Prestige Antibodies in immunohistochemistry applications, in organelle marker detection studies for sub- cellular localization and biomarker discovery studies. Download your complimentary copy at sigma.com/techaids . |
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