Prestige Antibodies^® in Immunohistochemistry

Immunohistochemistry (IHC) is the most widely used technique in histopathological diagnosis and research for detection of proteins in tissues and cells. Today, IHC can be applied in a high-throughput fashion for studying proteins by the use of Tissue Microarrays (TMAs). In the Human Protein Atlas Project, Prestige Antibodies have been used to analyze more than 8,000 human proteins using IHC and TMAs.1,2 All resulting tissue and cell images are publicly available on the Human Protein Atlas web portal (www.proteinatlas.org).3,4 In total, more than 500 high resolution IHC images from human tissue samples are presented for each Prestige Antibody. Each year protein expression and localization data of ~2,500 new proteins are added to the portal. By the end of 2014, a first draft of the localization of the full human proteome will be available.

Click here to download the Prestige Antibodies in Immunohistochemistry (627 Kb PDF) article.

Immunohistochemical stainings showing subcellular localization using Prestige Antibodies. Recognition of target antigen is represented by brown color.

A. Human rectum tissue showing membranous and/or cytoplasmic staining of glandular cells Anti-CDH1 (HPA004812)		B. Human testis tissue showing positive staining of spermatides and especially spermatozoa Anti-ODF2 (HPA001874)		C. Human small intestinal tissue showing strong positive staining of luminal surface Anti-SLC44A2 (HPA003228)
D. Human kidney tissue showing granular cytoplasmic staining of cells in tubuli Anti-ACADM (HPA006198)		E. Human gall bladder tissue showing structured golgi like positive staining of glandular cells Anti-GOLGA5 (HPA000992)		F. Human adrenal gland tissue showing a cytoplasmic microgranular pattern in cortical cells Anti-GLA (HPA000966)

References

1. Pontén F. et al. The Human Protein Atlas - a tool for pathology. J Pathology, 2008 216(4):387-93.
2. Kampf C. et al. Antibody-based tissue profiling as a tool in clinical proteomics. Clin. Proteomics, 2004, 1(3-4):285-300.
3. Uhlén M. et al. A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics. Mol Cell Proteomics, 2005, 4(12):1920-1932.
4. Berglund L. et al. A gene-centric human protein atlas for expression profiles based on antibodies. Molecular & Cellular Proteomics, 2008, 7:2019-2027.

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