Antibodies

Towards a Knowledge-based Human Protein Atlas Webinar

Presented by Dr. Mathias Uhlen, Royal Institute of Technology (KTH), Sweden

The human genome has approximately 20,000 protein-encoded genes. An import quest is to characterize the expression, localization, modification and function of the proteins encoded from each gene. A human protein atlas project has been initiated involving groups in Sweden, Korea, China and India using antibody-based protein profiling based on the information obtained from the sequencing of human genomes. The aim is to generate a fist draft on a whole-proteome level of protein localizations in human cells, tissues and organs, including various disease-related tissues. The version 7 of the Human Protein Atlas with sub-cellular localization data and expression data for all major human tissues and organs was launched in November 2010. A milestone has been achieved with the inclusion of expression data for more than 50% of the human protein-coded genes. The main new feature of the release is an attempt towards a knowledge-based portal, introducing the concept of annotated protein expression for paired antibodies, in which two or more independent antibodies are used to validate the staining pattern of each other. The new portal contains a summary page for every human gene. A new organ view including 46 tissues and organs and divided into 12 functional classes. A new cancer view including results from 20 different types of cancer. We have used the human protein atlas to study the global protein expression patterns in human cells, tissues and organs as well as a discovery tool to find potential biomarkers for cancer diagnostics.



5 videos
Total length: 40 minutes