Prestige Antibodies^® as Organelle Markers

The Human Protein Atlas Program has carefully selected three different human cell lines, A-431 epidermoid carcinoma, U-251 MG glioblastoma and U-205 osteosarcoma, for organelle mapping of the proteome. As Prestige Antibodies are studied by immunofluorescence (IF) staining, three well-characterized organelle markers for nuclei, microtubules and endoplasmic reticulum are used correspondingly as specific probes. The high-resolution confocal images are annotated based on sub-cellular localization. In addition, staining intensity and characteristics are also part of an overall assessment as well as comparison to literature (if available). All these factors contribute to a final reliability score publicly available on line. Among the Prestige Antibodies tested in immunofluorescence (IF), a set of specific antibodies is classified as organelle markers. These probes have been subjected to the well-established quality control by the HPA program, and are thus very well suited for sub-cellular localization studies by immunofluorescence (IF.)

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Intercellular Junctions HPA001636, Anti-TJP1 HPA001813, Anti-TJP2 Nucleus HPA002844, Anti-FBXO18 HPA000593, Anti-MECP2 HPA001894, Anti-GPKOW HPA006801, Anti-XRCC4 HPA006104, Anti-MYST4 Nucleoli HPA003436, Anti-CHX10 HPA007938, Anti-ZSCAN1 Nucleus without Nucleoli HPA008784, Anti-FUS HPA006149, Anti-FUBP1 HPA004776, Anti-AKAP8 Nuclear Envelope HPA008461, Anti-UNC84A HPA001209, Anti-UNC84B Golgi HPA000992, AntiGOLGA5 HPA001677, Anti-GOLPH4 Mitochondria HPA006181, Anti-MRPL40 HPA002932, Anti-TIMM9 HPA000866, Anti-SYNJ2BP HPA000898, Anti-HSPA9 Peroxisomes HPA007244, Anti-ACAA1 Cytoplasm HPA001290, Anti-MTHFD1 HPA001648, Anti-DDX3X HPA006731, Anti-USP10 Focal Adhesions HPA004835, Anti-ZYX HPA005724, Anti-VASP HPA001349, Anti-MYH6 Plasma Membrane HPA001672, Anti-SLC9A3R2 HPA003097, Anti-NF2 Endoplasmic Reticulum HPA003230, Anti-PDIA3 HPA003906, Anti-BCAP31 HPA003178, Anti-KTN1 Centrosomes HPA003647, Anti-DNAL4 Cytoskeleton-Microtubules HPA006376, Anti-TGFB111 Cytoskeleton-Actine Filaments HPA002925, Anti-FLNA HPA006035, Anti-ACTN1 Cytoskeleton-Intermediate Filaments HPA001762, Anti-VIM HPA007007, Anti-NES	Figure 1. Intercellular Junctions: Anti-TJP1 Cat. No. HPA001636		Figure 6. Cytoplasm: Anti-ATP-dependent RNA helicase DDX3X Cat. No. HPA001648
	Figure 2. Nuclease: Anti-F-box only protein 18 Cat. No. HPA002844		Figure 7. Focal Adhesions: Anti-Zyxin Cat. No. HPA004835
	Figure 3. Nucleoli: Anti-visual system homeobox 2 Cat. No. HPA003436		Figure 8. Endoplasmic Reticulum: Anti-Protein disulfide-isomerase A3 precursor Cat. No. HPA003230
	Figure 4. Nuclear Envelope: Anti-Sad1/unc-84-like protein 2 Cat. No. HPA001209		Figure 9. Centrosomes: Anti-Dynein light chain 4, axonemal Cat. No. HPA003647
	Figure 5. Mitochondria: Anti-Stress-70 protein, Mitochondria precursor Cat. No. HPA000898		Figure 10. Cytoskeleton-Microtubules: Anti-TGFB111 Cat. No. HPA006367

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