ACCUSPIN and Histopaque®

The iodinated aromatic compounds, originally devised for x-ray contrast applications, solve the more serious deficiencies of the other classes of gradient media. Iodinated gradient media have much lower osmolarities and viscosities than sucrose at any concentration. Polysaccharides such as Ficoll® are even more viscous than sucrose at all densities. Ionic gradient media, such as cesium chloride have higher densities and lower viscosities than other density gradient media. However, their use is restricted due to the high osmolarities and ionic strength which affect the hydration of osmotically sensitive particles and can disrupt or otherwise modify the integrity of biological particles.

Because of their positive properties, iodinated gradient media are used in a wide rage of applications. The structures of most iodinated compounds used as gradient media are based on tri-iodobenzoic acid with a hydrophilic group attached to increase their solubility. The first of these nonionic density gradients, iohexol (e.g., Nycodenz® and Histodenz), became available in the 1970s. Iohexol solutions are more dense at any given concentration than other gradient media types. This means that a lower media concentration is needed for any particular concentration, which minimizes the possibility of biological particles becoming dehydrated. Iohexol is nontoxic and not metabolized by mammalian cells.

Accuspin

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A0561 ACCUSPIN System-Histopaque®-1077 γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.
 
A1805 ACCUSPIN Tubes Sterile, 12 mL Capacity Polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 3 mL of density gradient.
Package contains 20 tubes
A2055 ACCUSPIN Tubes Sterile, 50 mL Capacity Polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of density gradient.
Package contains 10 tubes
A6929 ACCUSPIN System-Histopaque®-1077 γ-irradiated 12 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 3 mL of Histopaque 1077. Each tube will separate 3-6 mL of anticoagulated blood.
Package contains 40 prefilled tubes
A7054 ACCUSPIN System-Histopaque®-1077 γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.
Package contains 12 prefilled tubes

Histopaque

Sigma Life Science offers a complete line of products for the separation or extraction of leukocytes, viruses, DNA, RNA, organelles as well as many other applications. Featured in the product line is ACCUSPIN , a sterile, 2-chamber tube separated by a porous frit. Whole blood can be added directly to the ACCUSPIN tube without the risk of mixing with the Histopaque® -1077 contained in the lower chamber.

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10771 Histopaque®-1077 sterile-filtered, density: 1.077 g/mL
H8889 Histopaque®-1077 Hybri-Max liquid, sterile-filtered, BioReagent, suitable for hybridoma
10831 Histopaque®-1083 sterile-filtered, density: 1.083 g/mL
11191 Histopaque®-1119 sterile-filtered, density: 1.119 g/mL