Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Isocitrate Dehydrogenase (IDH) in Cancer Metabolism

Isocitrate dehydrogenase (IDH) catalyzes the conversion of isocitrate to a-ketoglutarate. In eukaryotes, there are three isozymes of IDH, the mitochondrial IDH2 and IDH3, and the cytoplasmic/peroxisomal IDH1. IDH1 and IDH2 mutations resulting in neomorphic enzymatic activity are found in certain cancers such as glioblastoma, acute myeloid leukemia, and colon cancer. This neoactivity shows a change in the substrate specificity resulting in the conversion of a-ketoglutarate to 2-hydroxyglutarate.

Antibodies Against Proteins Involved in the IDH Pathway

Product #

Description

Clone

Form

Gene Symbol (ID)

Species Reactivity

Application

Add to Cart

HPA044341 Anti-CIC antibody produced in rabbit polyclonal
affinity isolated antibody
human ... CIC(23152)
human
IF, IHC
HPA035248 Anti-IDH1 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... IDH1(3417)
human, mouse, rat
IHC
AMAB90578 Monoclonal Anti-IDH1 antibody produced in mouse CL0219, monoclonal
purified immunoglobulin
human ... IDH1(3417)
human
IF, IHC
HPA007831 Anti-IDH2 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... IDH2(3418)
rat, human, mouse
IF, IHC
WH0003418M1 Monoclonal Anti-IDH2 antibody produced in mouse 5F11, monoclonal
purified immunoglobulin
human ... IDH2(3418)
human
ELISA (i), IHC (p), WB
HPA019032 Anti-TET1 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... TET1(80312)
human
IF, IHC
SAB2501479 Anti-TET2 antibody produced in goat polyclonal
affinity isolated antibody
human ... TET2(54790)
bovine, human, mouse, canine, rabbit
ELISA (i), IHC
HPA050845 Anti-TET3 antibody produced in rabbit polyclonal
affinity isolated antibody
human ... TET3(200424)
human
IF, IHC

Assay Kits for Studying Isocitrate Dehydrogenase (IDH) Activity

Product #

Description

Add to Cart

MAK062 IDH Activity Assay Kit sufficient for 100 colorimetric tests
MAK061 Isocitrate Assay Kit sufficient for 100 colorimetric tests
MAK054 α-Ketoglutarate Assay Kit sufficient for 100 colorimetric or fluorometric tests

Gene Editing Tools For Studying the IDH Pathway

Product #

Product Name

Gene Symbol (ID)

Add to Cart

EHU110401 MISSION® esiRNA esiRNA human IDH1 (esiRNA1) human ... IDH1(3417)
EHU024791 MISSION® esiRNA esiRNA human TET2 (esiRNA1) human ... TET2(54790)
EHU097551 MISSION® esiRNA esiRNA human TET3 (esiRNA1) human ... TET3(200424)
EHU124501 MISSION® esiRNA esiRNA human TET3 (esiRNA2) human ... TET3(200424)
EHU053891 MISSION® esiRNA esiRNA human TET1 (esiRNA1) human ... TET1(80312)
EHU057381 MISSION® esiRNA esiRNA human CIC (esiRNA1) human ... CIC(23152)

Isocitrate Dehydrogenase (IDH) Proteins

Product #

Description

Add to Cart

I2411 Isocitrate Dehydrogenase 1 (NADP+) human recombinant, expressed in Sf9 cells, ≥90% (SDS-PAGE)

Isocitrate Dehydrogenase (IDH) Substrates and Reaction Products

Product #

Description

Add to Cart

H8378 D-α-Hydroxyglutaric acid disodium salt ≥95% (GC)
90790 L-α-Hydroxyglutaric acid disodium salt ≥98.0% (GC)
I1252 DL-Isocitric acid trisodium salt hydrate ≥93%
K2010 α-Ketoglutaric acid sodium salt BioUltra
58790 (+)-Potassium Ds-threo-isocitrate monobasic ≥98.0% (NT)