Mevalonate Pathway in Cancer

The mevalonate pathway is the central pathway of cholesterol biosynthesis resulting in mevalonate which is used for the synthesis of isoprenoids, and precursors of cholesterol. The rate-limiting step is the reduction of HMG-CoA by the enzyme HMG-CoA reductase, which is the pharmacological target of cholesterol-lowering statins. Isorenoids generated from mevalonate, farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP), are responsible for post-translational modification of proteins required for proliferation and differentiation, such as Ras and RhoGTPases(1).

1. Riganti, C. and M. Massaia. Inhibition of the mevalonate pathway to override chemoresistance and promote the immunogenic demise of caner cells. OncoImmunology 2013; 2:e25770.

Antibodies Against Proteins Involved in the Mevalonate Pathway

Product #

Description

Clone

Form

Gene Symbol (ID)

Species Reactivity

Application

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HPA028200 Anti-FDPS antibody produced in rabbit polyclonal
affinity isolated antibody
human ... FDPS(2224)
human
IF, IHC
HPA008338 Anti-HMGCR antibody produced in rabbit polyclonal
affinity isolated antibody
human ... HMGCR(3156)
human
IHC
HPA021147 Anti-MPO antibody produced in rabbit polyclonal
affinity isolated antibody
human ... MPO(4353)
human
IHC
HPA030646 Anti-PGGT1B antibody produced in rabbit polyclonal
affinity isolated antibody
human ... PGGT1B(5229)
rat, mouse, human
IF, IHC
WH0005229M2 Monoclonal Anti-PGGT1B antibody produced in mouse 5E4, monoclonal
purified immunoglobulin
human ... PGGT1B(5229)
mouse, human, rat
ELISA (i), IF, WB

Assay Kits for Analyzing the Mevalonate Pathway

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MAK043 Cholesterol Quantitation Kit sufficient for 100 colorimetric or fluorometric tests
CS1090 HMG-CoA Reductase Assay Kit sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)
MAK069 MPO Fluorometric Activity Assay Kit sufficient for 100 fluorometric tests

Gene Editing Tools For Studying the Mevalonate Pathway

Product #

Product Name

Gene Symbol (ID)

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EHU112171 MISSION® esiRNA esiRNA human PGGT1B (esiRNA1) human ... PGGT1B(5229)
EHU116031 MISSION® esiRNA esiRNA human FDPS (esiRNA1) human ... FDPS(2224)
EMU041131 MISSION® esiRNA esiRNA targeting mouse Pggt1b (esiRNA1) mouse ... Pggt1b(225467)
EHU079561 MISSION® esiRNA esiRNA human HMGCR (esiRNA1) human ... HMGCR(3156)
EMU034221 MISSION® esiRNA esiRNA targeting mouse Hmgcr (esiRNA1) mouse ... Hmgcr(15357)
EMU187691 MISSION® esiRNA esiRNA targeting mouse Fdps mouse ... Fdps(110196)

Inhibitors and Activators of the Mevalonate Pathway

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A4978 Alendronate sodium trihydrate ≥97% (NMR), powder
P5248 Etidronate disodium hydrate ≥97% (NMR), solid
G5294 GGTI-2133 ≥98% (HPLC), solid
G5169 GGTI 298 trifluoroacetate salt hydrate ≥90% (HPLC), film
I5784 Ibandronate sodium salt ≥97% (NMR), solid
M2537 Mevastatin ≥95% (HPLC), powder
M2147 Mevinolin from Aspergillus sp. ≥98% (HPLC)
P2371 Pamidronate disodium salt hydrate ≥95% (NMR), solid
P4498 Pravastatin sodium salt hydrate ≥98% (HPLC), powder
S6196 Simvastatin ≥97% (HPLC), solid
S4194 SR 12813 ≥98%, solid
SML0223 Zoledronic acid monohydrate ≥98% (HPLC)

Key Mevalonate Metabolites Implicated In Cancer

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Description

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277541 trans,trans-Farnesol 96%
F6892 Farnesyl pyrophosphate ammonium salt methanol:ammonia solution, ≥95% (TLC)
G3278 Geranylgeraniol ≥85% (GC)
G6025 Geranylgeranyl pyrophosphate ammonium salt solution, ≥95% (TLC), ~1 mg/mL in methanol: NH4OH (7:3)
44714 (S)-Mevalonic acid lithium salt ≥96.0% (GC)
90469 (RS)-Mevalonic acid lithium salt ≥96.0% (GC)
68519 (R)-(−)-Mevalonolactone ≥90.0% (GC)

Proteins Involved in the Mevalonate Pathway

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H7039 3-Hydroxy-3-methylglutaryl-CoA reductase human solution
M6908 Myeloperoxidase from human leukocytes lyophilized powder, ≥50 units/mg protein