Intestinal Drug Transporter Knockout Models

Caco-2 cells are human intestinal epithelium cells that express multiple drug transporters and grow in a tight homogenous monolayer. Caco-2 cells are commonly used to predict human intestinal drug permeability and to investigate drug efflux.
To aid in the investigation of drugs¢ absorption across the human intestinal epithelium, Sigma has generated transporter knockout (KO) Caco-2 cell lines using CompoZr Zinc Finger Nuclease (ZFN) technology. Sigma offers Caco-2 control cells and transporter KO cell lines as Assay Ready Plates.

Features and Benefits:
  • Ease of use: Supplied to the cusstomer on day 16 of the 21 day culturing process, in either 24 well or 96 well transwell plate format, to reduce hands on time and permit easy manual or automated processing
  • Reproducible, standardized results: Products are produced in a dedicated facility under a rigorous quality management system to ensure reproducible results
  • Highly predictive: Transporter KO cell lines eliminate the ambiguity from non-specific substrates and inhibitors
  • Meet regulatory guidelines: The drug transporters targeted include those referenced in the regulatory guidance for investigating drug-drug interactions (DDI) from the US Food and Drug Administration (FDA), the European Medicines Agency (EMA), and the Japanese Ministry of Health, Labour, and Welfare (MHLW): MDR1 (P-gp), BCRP, MRP2; and those of emerging clinical importance: MRP1, MRP3, MRP4, MRP5, MRP6 and MRP7

Assay Ready Plates

Product #

Description

Add to Cart

MTOX1000P24 Caco-2 Control Cells one assay ready, 24 well plate
MTOX1000P96 Caco-2 Control Cells one assay ready, 96 well plate
MTOX1001P24 MDR1 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1001P96 MDR1 Knockout Caco-2 Cells one assay ready, 96-well plate
MTOX1002P24 BCRP Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1002P96 BCRP Knockout Caco-2 Cells one assay ready, 96-well plate
MTOX1003P24 MRP2 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1003P96 MRP2 Knockout Caco-2 Cells one assay ready, 96-well plate
MTOX1004P24 MDR1/BCRP Double Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1004P96 MDR1/BCRP Double Knockout Caco-2 Cells one assay ready, 96-well plate
MTOX1005P24 MDR1/MRP2 Double Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1005P96 MDR1/MRP2 Double Knockout Caco-2 Cells one assay ready, 96-well plate
MTOX1006P24 BCRP/MRP2 Double Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1006P96 BCRP/MRP2 Double Knockout Caco-2 Cells one assay ready, 96-well plate
MTOX1092P24 MRP1 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1092P96 MRP1 Knockout Caco-2 Cells one assay ready, 96 well plate
MTOX1093P24 MRP3 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1093P96 MRP3 Knockout Caco-2 Cells one assay ready, 96 well plate
MTOX1094P24 MRP4 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1094P96 MRP4 Knockout Caco-2 Cells one assay ready, 96 well plate
MTOX1095P24 MRP5 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1095P96 MRP5 Knockout Caco-2 Cells one assay ready, 96 well plate
MTOX1097P24 MRP7 Knockout Caco-2 Cells one assay ready, 24 well plate
MTOX1097P96 MRP7 Knockout Caco-2 Cells one assay ready, 96 well plate