EdU Cell Proliferation Detection Kits

Rapid detection in a variety of formats

Baseclick assays provide an efficient method for fluorescence detection of replicating DNA. The modified nucleoside EdU is added to live cells and is incorporated into replicating DNA. Cu-induced click chemistry allows rapid attachment of fluorescent probes to the EdU. This provides for a quantitative way to monitor cells that are proliferating. The assays are available in various formats for microscopy imaging, flow cytometry, high throughput screening, and for in vivo experiments. Four different fluorescent probes with excitation near 488, 555, 594, and 647 are available so that the readout can be multiplexed with other fluorescent probes.

Benefits of Baseclick Proliferation Assays

  • Direct Detection of Nucleic Acids - The Baseclick assay provides for a direct measurement of nucleic acid content that is more specific for proliferation than dyes that detect metabolism or protein content.
  • Faster Than BrdU assays - EdU detection requires less steps than is needed with antibody detection in the anti-BrdU assay, only requiring a total time of ~1 1/2 hr (30 min for the click ligation).
  • Low Toxicity - EdU incorporation into DNA is an effective replacement for BrdU showing similar low toxicity in cell lines and complex organisms.
  • High Efficiency - The reaction and fluorescent dye chemistry have been optimized to provide a strong fluorescent signal. This allows high sensitivity of proliferating cells even when using a plate reader.
  • Works in Many Model Organisms - EdU incorporation has been used in the following model organisms: mouse, rat, C. elegans, cricket, chicken, zebra fish larva, and Arabidopsis.

For more information visit, Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time
To learn more from the literature visit, Publications using EdU Cell Proliferation Assays from Baseclick

Imaging Kits

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BCK-EDU488 EdU-Click 488 sufficient for 100 assays
BCK-EDU555 EdU-Click 555 sufficient for 100 assays
BCK-EDU594 EdU-Click 594 sufficient for 100 assays
BCK-EDU647 EdU-Click 647 sufficient for 100 assays

Flow Cytometry Kits

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BCK-FC488-100 EdU Flow Cytometry Kit 488 sufficient for 100 assays
BCK-FC488-50 EdU Flow Cytometry Kit 488 sufficient for 50 assays
BCK-FC555-100 EdU Flow Cytometry Kit 555 sufficient for 100 assays
BCK-FC555-50 EdU Flow Cytometry Kit 555 sufficient for 50 assays
BCK-FC594-100 EdU Flow Cytometry Kit 594 sufficient for 100 assays
BCK-FC594-50 EdU Flow Cytometry Kit 594 sufficient for 50 assays
BCK-FC647-100 EdU Flow Cytometry Kit 647 sufficient for 100 assays
BCK-FC647-50 EdU Flow Cytometry Kit 647 sufficient for 50 assays

High Throughput Screening Kits

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BCK-HTS488-2 EdU HTS Kit 488 sufficient for 200 assays
BCK-HTS488-20 EdU HTS Kit 488 sufficient for 2000 assays
BCK-HTS488-4 EdU HTS Kit 488 sufficient for 400 assays
BCK-HTS555-2 EdU HTS Kit 555 sufficient for 200 assays
BCK-HTS555-20 EdU HTS Kit 555 sufficient for 2000 assays
BCK-HTS555-4 EdU HTS Kit 555 sufficient for 400 assays

In Vivo Kits

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BCK488-IV-IM-L In Vivo EdU Click Kit 488 sufficient for 100 assays
BCK488-IV-IM-M In Vivo EdU Click Kit 488 sufficient for 100 assays
BCK488-IV-IM-S In Vivo EdU Click Kit 488 sufficient for 100 assays
BCK555-IV-IM-L In Vivo EdU Click Kit 555 sufficient for 100 assays
BCK555-IV-IM-M In Vivo EdU Click Kit 555 sufficient for 100 assays
BCK555-IV-IM-S In Vivo EdU Click Kit 555 sufficient for 100 assays
BCK594-IV-IM-L In Vivo EdU Click Kit 594 sufficient for 100 assays
BCK594-IV-IM-M In Vivo EdU Click Kit 594 sufficient for 100 assays
BCK594-IV-IM-S In Vivo EdU Click Kit 594 sufficient for 100 assays
BCK647-IV-IM-L In Vivo EdU Click Kit 647 sufficient for 100 assays
BCK647-IV-IM-M In Vivo EdU Click Kit 647 sufficient for 100 assays
BCK647-IV-IM-S In Vivo EdU Click Kit 647 sufficient for 100 assays
BCK488-IV-FC-L In Vivo EdU Flow Cytometry 50 Kit 488 sufficient for 50 assays
BCK488-IV-FC-M In Vivo EdU Flow Cytometry 50 Kit 488 sufficient for 50 assays
BCK488-IV-FC-S In Vivo EdU Flow Cytometry 50 Kit 488 sufficient for 50 assays
BCK555-IV-FC-L In Vivo EdU Flow Cytometry Kit 555 sufficient for 50 assays
BCK555-IV-FC-M In Vivo EdU Flow Cytometry Kit 555 sufficient for 50 assays
BCK555-IV-FC-S In Vivo EdU Flow Cytometry Kit 555 sufficient for 50 assays
BCK594-IV-FC-L In Vivo EdU Flow Cytometry Kit 594 sufficient for 50 assays
BCK594-IV-FC-M In Vivo EdU Flow Cytometry Kit 594 sufficient for 50 assays
BCK594-IV-FC-S In Vivo EdU Flow Cytometry Kit 594 sufficient for 50 assays
BCK647-IV-FC-L In Vivo EdU Flow Cytometry Kit 647 sufficient for 50 assays
BCK647-IV-FC-M In Vivo EdU Flow Cytometry Kit 647 sufficient for 50 assays
BCK647-IV-FC-S In Vivo EdU Flow Cytometry Kit 647 sufficient for 50 assays
BCK488-IV-HTS-L In Vivo EdU HTS 2x96 Kit 488 sufficient for 200 assays
BCK488-IV-HTS-M In Vivo EdU HTS 2x96 Kit 488 sufficient for 200 assays
BCK488-IV-HTS-S In Vivo EdU HTS 2x96 Kit 488 sufficient for 200 assays
BCK555-IV-HTS-L In Vivo EdU HTS 2x96 Kit 555 sufficient for 200 assays
BCK555-IV-HTS-M In Vivo EdU HTS 2x96 Kit 555 sufficient for 200 assays
BCK555-IV-HTS-S In Vivo EdU HTS 2x96 Kit 555 sufficient for 200 assays