PKH and CellVue® Fluorescent Cell Linkers

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects. Patented cell linker technology incorporates aliphatic reporter molecules into the cell membrane lipid bilayer by selective partitioning. PKH2 and PKH67 are green fluorochromes with excitation (490 nm) and emission (504 nm) similar to fluorescein. PKH26, is a red fluorochrome which, has excitation (551 nm) and emission (567 nm) characteristics compatible with rhodamine or phycoerythrin detection systems. PKH26 may also be excited by the 488 nm emission of an argon-ion laser. CellVue® Claret is a far-red fluorochrome which is independent of pH in physiologic ranges, and has excitation (655 nm) and emission (675 nm) characteristics compatible with a red diode laser. The linkers are physiologically stable and show little to no toxic side-effects on cell systems. Labeled cells retain both biological and proliferative activity, and are ideal for cell tracking and cell-cell interaction studies.

View the JoVE video: Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
PKH26-labeled young migrating neurons invading the developing cortex. (Photo courtesy of H. Wichte...
Confocal image of a Leishmania donovani promastigote labeled with PKH26 and expressing GFP....
Standard Protocol for PKH Dye Labeling

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details CellVue® Claret Far Red Fluorescent Cell Linker Kit
GLDIL Diluent A For general membrane labeling
PCLDIL Diluent B for Phagocytic cell labeling Distributed for Phanos Technologies
CGLDIL Diluent C for General Membrane Labeling
PKH26GL PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling
PKH26PCL PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling
MINI26 PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
P7458 PKH26 Reference Microbeads
PKH2GL PKH2 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling
PKH67GL PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling Distributed for Phanos Technologies
MIDI67 PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling
MINI67 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling