Caspases Enzymes

Caspase Cascade
Apoptosis or programmed cell death is triggered by a variety of stimuli, including cell surface receptors like FAS, the mitochondrial response to stress, and factors released from cytotoxic T cells. The caspases comprise a class of cysteine proteases, many members of which are involved in apoptosis. The caspases convey the apoptotic signal in a proteolytic cascade, with caspases cleaving and activating other caspases that in turn activate enzymes that subsequently degrade cellular targets that lead to cell death. The activating caspases include caspase-8 and caspase-9. Caspase-8 is the initial caspase activated in response to receptors with a death domain that interacts with FADD. The mitochondrial stress pathway begins with the release of cytochrome c from mitochondria, which then interacts with Apaf-1, causing self-cleavage and activation of caspase-9. The effector caspases, caspase-3, -6, -7 are downstream of the activator caspases and act to cleave various targets. Activation of caspase-3 and caspase-7 can be blocked by IAP proteins. Granzyme B and perforin, proteins released by cytotoxic T cells, induce apoptosis in target cells by forming transmembrane pores and triggeringapoptosis, perhaps through cleavage of caspases. Caspase-independent mechanisms of granzyme B-mediated apoptosis have been suggested. In addition, caspase-activated DNAse (CAD) may be activated through the cleavage of its associated inhibitor ICAD. CAD is then able to interact with components such as topoisomerase II (Topo II) to condense chromatin and lead to DNA fragmentation.
The Caspase Cascade

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C5482 Caspase 1 human recombinant, expressed in E. coli, ≥5000 units/mg protein
C2854 Caspase 2 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, >1000 units/mg protein
C1224 Caspase 3 human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein
C6357 Caspase 4 human recombinant, expressed in E. coli, 5,000 units/mg protein
C6482 Caspase 5 human recombinant, expressed in E. coli, 5,000 units/mg protein
C2979 Caspase 7 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, ≥1,500 units/mg protein
C1099 Caspase 8 human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine tagged), buffered aqueous solution, ≥1,000 units/mg protein
C8726 Caspase 9 human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein
C6607 Caspase 10 human >90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, 8,000 units/mg protein (Bradford)
C8232 Caspase Combo II, Type 2, 3, and 7 human >90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder