Biochemicals

Detection – Superior Toolbox for Fluorescence and Chemiluminescence Applications

Image: Application of Atto 488 Phalloidin

Confocal Laser Scanning Micrograph (CLSM) of human peripheral blood monocytes differentiated to macrophages, and infected with a red fluorescent Serratia liquefaciens. A macrophage try to capture bacteria by means of a long pseudopod. Bacteria seem to walk along this structure.

Fixed cells were permeabilized with Triton X-100. Phalloidin-Atto 488 (Sigma, Cat No. 49409) binds polymerized F-actin, used to identify actin filaments and fibers. Preparations were mounted in Fluoroshield- mounting medium containing DAPI (Sigma Aldrich). Series of optical sections were obtained with a NIKON A1R confocal scanning laser microscope equipped with a Nikon A1 digitalcamera, and a 403nm, 488nm, 561nm lasers.

Image was kindly provided by Prof. Jose Ramos Vivas, Hospital Universitario Marqués de Valdecilla & Instituto de Formación e Investigación Marqués de Valdecilla-IFMAV.

Sensitive and flexible detection techniques are of increasing importance and popularity in biological research. Luminescent techniques enable highest sensitivity and selectivity detection even within cells and tissue sections. Luminescence monitoring is usually rather simple and easily automated. Our product range includes fluorescent labels, conjugates, substrates, indicators and probes, electrophoresis stains, membrane probes, microparticles, general dyes, and embedding media.

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