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Spectral Fluorescence Standard Kit

The Spectral Fluorescence Standard Kit (Cat. No. 69336) enables a simple characterization of the relative spectral responsivity and the long-term stability of the emission channel of fluorescence instruments under routine measurements conditions providing the basis for an improved comparability of fluorescence measurements and eventually standardization.

Application of the Emission Correction Curve


Bottom: Certified normalized corrected emission
spectra (solid lines; (lc(λem)) of the kit components
and uncorrected, i.e. instrumentdependent
emission spectra (dashed lines; lu (λem)),
measured with the instrument to be calibrated.

Middle: Incividual quotient QF00x = lcF00x (λem)/
luF00x (λem) for each kit dye equaling 1/s(λ) of
the instrument to be calibrated within the spectral
region of the respective fluorescence standard.

Top: Combined emission correction curve 1/s(λ)
(solid pik line) calculated from the statistically
weighted QF00x as well as its reciprocal s(λ)
(black solid line).

Corrected emission spectra are obtained by
multiplication of measured spectra by the output
of LINKCORR, i.e., 1/s(λ).



Certified Properties

Normalized corrected emission spectra of BAM-F001 – BAM-F005 in ethanol for T=25 °C. The emission spectra are traceable to the spectral radiance realized and disseminated in Germany by the Physikalisch-Tecnische Bundesanstalt (PTB).

The Calibration Kit has been tested in an interlaboratory comparison of the National Metrological Institutes NIST (National Institutes of Standards and Technology, USA), NRC (National Research Council, Canada), PTB (Physikalisch-technische Bundesanstalt, Germany), and BAM (Federal Institute for Material Testing) employing two different dye concentrations and two different measurement geometries, i.e., 0°/90° and 45°/0°.

Components

  • CD with Improved Software LINKCORRWIN V.1.1.0.0. Developed by BAM for the data evaluation. Version1.1.0.0 only performs well in conjunction BAM-F002a. The CD contains also an instructions for use of BAM-F001 – BAM-F005 and LINKCORRWIN.
  • Five Spectral Florescence Standards BAM-F001 (Cat. No. 72594), BAM-F002a (Cat. No. 07021), BAM-F003 (Cat. No. 96158), BAM- F004 (Cat. No. 74245), and BAM-F005 (Cat. No. 94053) ready-made from Sigma-Aldrich GmbH, which cover the spectral region of 300 nm to 770 nm as a set.
  • 100 Ethanol (Cat. No. 34923) Addition of aliquots of 10 mL of ethanol to each solid dye yields a solution that can be measured without additional dilution steps.
  • BAM Certificate of the normalized corrected emission spectr 

 

Certified Reference Materials

  • For the determination of the relative spectral responsivity s (λ) of fluorescence instruments.
    1/s (λ) is termed emission correction curve. Both can be determined by LINKCORRWIN.
  • For the determination of corrected, i.e., instrument-independent emission spectra that are comparable across instruments.
  • For the characterization of the long-term stability of the emission channel of fluorescence instruments. Fluorescence spectra that are corrected accordingly do not contain contributions from aging of optical components in the emission channel.

 

References
(1) Hollandt, J.; Taubert, R.D.; Seidel, J.; Resch-Genger, U.; Gugg-Helminger, A.; Pfeifer, D.; Monte, C.J. Fluoresc. 2005, 15, 311.
(2) Resch-Genger, U.; Pfeifer, D.; Pilz, W.; Monte, C.; Hoffmann, A.; Spieles, M.; Rurack, K.; Hollandt, J.; Taubert, D.; Schonenberger, B.; Nording, P. J.
      Fluoresc, 2005, 15, 325.
(3) Resch-Genger, U.; Hoffmann, K.; Nietfeld, W.: Engel, A.; Neukammer, J.; Nietschke, R.; Ebert, B.; Macdonald, R. J. Fluoresc. 2005, 15, 347.
(4) Hoffmann, K.; Monte, C.; Pfeifer, D.; Resch-Genger, U. G.I.T. Laboratory Journal 6, 2005, 3, 18. (5) Monte, C.; Pilz, W.; Resch-Genger, U. Proc.SPIE 2005, 5880, 599019-1.
(6) Hoffmann, K.; Monte, C.; Pfeifer, D.; Resch-Genger, U. G.I.T. Laboratory Journal 2005, 6, 29.
(7) Monte, C.; Resch-Genger, U.; Pfeifer, D.; Taubert, R.D.; Hollandt, J. Metrologia 2009, 16, 589.
(8) Monte, C.; Hoffmann, K.; Pfeifer, D.; Hoffmann, A.; Resch-Genger, U. J Fluoresc. 2006, 16, 441.